川乌HPLC指纹图谱的研究

HPLC Fingerprint of Radix Aconiti

目的:建立不同产地川乌药材的高效液相指纹图谱。方法:实验以Phenemonex Gemini-NX C18为色谱柱,流动相乙腈-0.2%乙酸(浓氨水调pH 10.00),采用梯度洗脱,流速0.8 mL.min-1,柱温35℃,检验波长235 nm,洗脱时间75 min。结果:建立了川乌药材HPLC指纹图谱共有模式,标定了12个共有峰,鉴别出6个色谱峰的归属,指纹图谱的相似度基本都在92%以上,通过系统聚类分析,10批不同产地川乌药材可分为3类。结论:川乌指纹图谱的特征性及专属性强,可结合含量测定用于全面控制川乌的质量。

Objective：To establish a method for HPLC-fingerprint of Radix Aconiti from different areas. Method： The fingerprint for Radix Aconiti from different areas was established by HPLC. Gemini-NX CIs column was used at 35 ℃ with acetonitrile-0.2% acetic acid （adjusted to pH 10. 0 with ammonia water） as mobile phase by gradient eluting, the flow rate was set at 0. 8 mL·min -1, detection wavelength was set at 235 nm and eluting period was 75 rain. Result： HPLC-fingerprint of R. aconiti from different areas was established, which contained 12 common peaks, and six peaks in the fingerprint were identified, most of the similarities were over 92% , 10 batches of R. aconiti from different areas were classified into three groups based on hierarchical cluster. Conclusion ： Fingerprint established was characteristic and specific, combined with the determination it could be used for the quality control of Radix Aconiti.