HPLC测定不同产地掌叶半夏中腺苷的含量

Determination of Content of Adenosine in Pinellia pedatisecta from Different Districts by HPLC

目的:采用HPLC法建立掌叶半夏中腺苷的含量测定方法。方法:采用KromasilODS-C18(4.6 mm×200 mm,5μm)色谱柱,流动相为甲醇-水(15∶85),流速1 mL.min-1,柱温25℃,检测波长260 nm。方法:腺苷在9.84～78.72 mg.L–1线性关系良好(r=0.999 8),平均加样回收率为99.01%,RSD 1.94%(n=6)。结果:6个不同产地掌叶半夏中腺苷含量大小为:山东菏泽>河北安国>陕西汉中>河北保定>安徽亳州>湖南浏阳。结论:该方法操作简便,结果可靠,重复性好,可作为掌叶半夏药材的质量控制方法之一。

Objective ： To establish an HPLC method to determine the content of adenosine in PineUia peda- tisecta from different districts. Method： Experiment was carried out on Kromasil C18（4.6mm×200mm,5μm） column with mobile phase consisted of methanol-water （ 15 ： 85 ） at a flow rate of 1.0 mL·min^-1. The detection wavelength was at 260 nm and the column temperature was at 25 ℃. Result： The linear range of adenosine was 9. 84-78.72 mg· L^- 1 （r = 0. 999 7 ）. The average recovery was 99.01% , RSD 1.94% （ n = 6）. The adenosine content of P. pedatisecta from different districts ranged in order was as follows ： Heze 〉 Anguo 〉 Hanzhong 〉 Baoding 〉 Bozhou 〉 Liuyang. Conclusion ： The method is quick ; simple and reproducible for the determination of adenosine in P. pedatisecta.