HPLC测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸

Determination of Protocatechuic Acid,Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC

目的:建立桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸的高效液相测定方法。方法:采用Hypersil ODS2 C18(4.6mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,流速1.0 mL.min-1,柱温为20℃,检测波长258,327 nm。结果:原儿茶酸、绿原酸和咖啡酸分别在0.032 2～0.161 0μg,0.125 6～1.256μg和0.035 1～0.351μg呈良好线性关系;平均回收率分别为102.1%、101.1%和101.5%。结论:该法操作简便,分离效果理想,可用于测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸的测定。

Objective：To establish the methods for determining protocatechuic acid, chlorgenic acid and caffeic acid in Sangye dispensing granules by HPLC. Method： The column of Hypersil ODS2 Cls column （4.6mm×250mm,5μm） was used. The mobile phase consisted of acetonitrile-0. 1% phosphoric acid with gradient elution. The flow rate was 1.0 mL·min^-1. The column temperature was at 20℃ , the detection wavelength was at 258 nm, 328 nm. Result： The average recovery of protocatechuic acid, chlorgenic acid and caffeic acid was 102. 1%, 101.1% and 101.5% respectively. Protocatechuic acid, chlorgenic acid and caffeic acid were linear in the range of 0. 032 2- 0. 161 0 μg, 0. 125 6-1. 256 μg and 0. 035 1-0. 351 μg respectively. Conclusion： The method is simple, accurate and can used to determine the content of the protocatechuic acid in mulberry formula granules.