灵芝多糖对阿尔茨海默病模型大鼠海马内突触及突触素表达的影响

Effects of Ganoderma lucidum Polysaccharides on Synapsis and Hippocampal Synaptophysin Expression in Alzheimer's Rats Model

目的:观察灵芝多糖(GLP)对阿尔茨海默病(AD)模型大鼠海马内突触及突触素表达的影响。方法:清洁级雄性SD大鼠,双侧海马内一次性注射β-淀粉样多肽25～35片段(Aβ25～35)制作大鼠AD模型;ip不同浓度的灵芝多糖溶液进行治疗,连续7 d,每天1次;Morris水迷宫检测动物学习记忆能力,采用免疫组织化学染色法、透射电镜技术结合图象分析方法比较各组大鼠海马CA1,CA3区突触素表达、突触的数密度和面密度变化。结果:与模型组相比,灵芝多糖75,50 mg.kg-1能显著降低大鼠水迷宫实验的平均潜伏期,升高跨越平台次数;亦能显著升高大鼠海马突触素的表达(P<0.01)及突触的数密度(Nv)和面密度(Sv)(P均<0.01)。结论:灵芝多糖能显著升高老年性痴呆(阿尔茨海默病)大鼠海马内降低的突触素/突触,此可能是增强大鼠学习记忆产生抗痴呆作用的机制之一。

Objective： To explore effects of Ganoderma lucidum polysaccharides （GLP） on synapsis and hippocampal synaptophysin expression in AD-rats. Method： AD-rats induced by Aβ25-35 were treated with different concentrations of GLP for 7 days. The Morris water maze was applied to validate the learning and memory ability in rats. The expression of synaptophysin and the alleosis of synaptic stereology were detected on tissues of CA1 and CA3 regions in hippocampus by immunohistochemitry method, transmission electron microscope （TEM） and imageanalysis system. Result： Compared with the model group, GLP （75,50 mg· kg^-1） decreased the average delitescence of rats in Morris water maze, and GLP increased the synaptophysin expression levels （P 〈 0.01 ） and numerica density （ Nv）, surface density （Sv） （ P 〈 0. 01, P 〈 0. 01 ）. Conclusion ： GLP improves the learning ability in AD-rats. Reversal of synaptophysin and Nv, Sv on hippocampus could be contributed to the mechanism.