骨碎补的木脂素和黄酮类成分研究

Lignans and flavonoids from rhizome of Drynaria fortunei

目的研究骨碎补Drynaria fortunei的化学成分。方法采用硅胶、ODS、Sephadex LH-20柱色谱及半制备液相色谱等技术分离纯化,根据理化性质及波谱数据鉴定化合物的结构。结果从骨碎补中分离得到14个化合物,包括3个木脂素类(1～3)和8个黄酮类化合物(4～11),其结构经1H-NMR1、3C-NMR、2D-NMR、ESI-MS等波谱学方法分别鉴定为(7′R,8′S)-二氢脱氢二松柏基醇4′-O-β-D-葡萄糖苷(1)、落叶松脂素4′-O-β-D-吡喃葡萄糖苷(2)、(?)-secoisolariciresinol 4-O-β-D-gluco-pyranoside(3)、北美圣草素(4)、eriodictyol 7-O-β-D-glucopyranoside(5)、新北美圣草苷(6)、柚皮苷(7)、木犀草素(8)、木犀草素7-O-β-D-葡萄糖苷(9)、木犀草素8-C-β-D-葡萄糖苷(10)、2′,4′-二羟基二氢查耳酮(11)、麦芽酚3-O-β-D-葡萄糖苷(12)、β-谷甾醇(13)、β-胡萝卜苷(14)。结论化合物1～3、11为首次从水龙骨科植物中分离得到,化合物5、8～10为首次从槲蕨属植物中分离得到,并发现化合物1在常温下存在旋转异构现象。

Objective To study the chemical constituents from the rhizome of Drynaria fortunei.Methods Silica gel,ODS,Sephadex LH-20 column chromatography,and semi-preparative HPLC were used to isolate pure compounds.The compounds were identified on the basis of their physicochemical properties and spectroscopic data.Results Fourteen compounds were isolated from the rhizome of D.fortunei,including three lignans（1—3） and eight flavonoids（4—11）.By spectroscopic techniques,such as 1H-NMR,13C-NMR,and ESI-MS,these compounds were identified as（7′R,8′S）-dihydrodehydrodiconiferyl alcohol 4′-O-β-D-glucopyranoside（1）,lariciresinol 4′-O-β-D-glucopyranoside（2）,（？）-secoisolariciresinol 4-O-β-D-glucopyranoside（3）,eriodictyol（4）,eriodictyol 7-O-β-D-glucopyranoside（5）,neoeriocitrin（6）,naringin（7）,luteolin（8）,luteolin 7-O-β-D-glucopyranoside（9）,luteolin 8-C-β-D-glucopyranoside（10）,2′,4′-dihydroxydihydrochalcone（11）,maltol 3-O-β-D-glucopyranoside（12）,β-sitosterol（13）,and daucosterol（14）.Conclusion Compounds 1—3,and 11 are isolated from the plants in Polypodiaceae family for the first time,and compounds 5 and 8—10 are isolated from the plants of Drynaria（Bory） J.Sm.for the first time.Compound 1 is present as rotamers at room temperature.