晶帽石斛化学成分研究

Chemical constituents of Dendrobium crystallinum

目的进一步对兰科石斛属植物晶帽石斛Dendrobium crystallinum的化学成分进行研究。方法应用硅胶、Sephadex LH-20、ODS、MCI柱色谱等方法进行分离纯化,根据理化性质和波谱数据鉴定化合物结构。结果从晶帽石斛醋酸乙酯和正丁醇部分共分离并鉴定了10个化合物,分别为4′-羟基-3,3′,5-三甲氧基联苄(1)、5′-羟基-3,3′,4-三甲氧基联苄(2)、3,3′-二羟基-5-甲氧基联苄(即山药素III)(3)、3,4′-二羟基-5-甲氧基联苄(4)、山柰酚(5)、6″-O-(3′′′-羟基-3′′′-甲基戊二酰)牡荆苷(6)、乌苏酸(7)、麦角甾-7,22-二烯-3β,5α,6β-三醇(8)、尿苷(9)、丁香脂素(10)。结论所有化合物均为首次从该植物中分离得到,其中化合物6、9为首次从兰科植物中分离得到,化合物2、8为首次从石斛属植物中分离得到。

Objective To study the constituents of the stem in Dendrobium crystallinum.Methods Compounds were isolated and purified by silica gel,Sephadex LH-20,ODS,and MCI column chromatography.Their structures were identified by physicochemical properties and spectral analyses.Results Ten compounds were obtained and identified as 4′-hydroxy-3,3′,5-trimethoxybibenzyl（1）,5′-hydroxy-3,3′,4-trimethoxybibenzyl（2）,3,3′-dihydroxy-5-methoxybibenzyl（batatasin III）（3）,3,4′-dihydroxy-5-methoxybibenzyl（4）,kaempferol（5）,6″-O-（3′′′-hydroxy-3′′′-methylglutaroyl） vitexin（6）,ursone（7）,ergosta-7,22-diene-3β,5α,6β-triol（8）,uridine（9）,and（＋）-syringaresinol（10）.Conclusion All these compounds are isolated from this plant for the first time,compounds 6 and 9 are obtained from the plants of Orchidaceae for the first time,and compounds 2 and 8 are obtained from the plants of Dendrobium Sw.for the first time.