制川乌白芍合煎微波提取工艺研究

Optimization of microwave-assistant extraction technology for codecoction of Aconiti Radix Cocta coupled with Paeoniae Radix Alba

目的优选微波法提取制川乌配伍白芍中有效成分乌头总生物碱和芍药苷的工艺条件。方法采用单因素结合正交设计法,优选出65%乙醇为提取溶媒,进一步考察了微波功率、微波辐射时间、乙醇体积分数及料液比4个因素,用紫外可见分光光度法测定乌头总生物碱,采用HPLC法测定芍药苷,并以其量及浸膏得率作为评价指标。结果以65%乙醇为提取溶媒,在微波功率800 W,微波辐射时间为0.5 h,固液比为1∶10时乌头总生物碱和芍药苷的提取量最高且浸膏得率最低。结论微波提取时间短,有效成分提取率高,此方法是一种有发展潜力的工艺。

Objective To optimize the microwave extraction（ME） technology for extraction of total aconitum alkaloids and paeoniflorin in co-decoction of Aconiti Radix Cocta coupled with Paeoniae Radix Alba..Methods Orthogonal test with single factor,as well as ME method was used,65% ethanol was optimized as extraction solvent,the effects of four factors,such as the microwave power,the radiation time,the solvent consumption,and ethanol concentration in microwave extraction process were investigated.The contents of total aconitum alkaloids were determined by UV-visible spectrophotometry and the paeoniflorin by HPLC by taking the contents and extract yield as evaluation indexes.Results The optimum conditions of extraction were as follows： 65% ethanol as extracting solvent,the microwave power was 800 W,the radiation time was 0.5 h,the proportion of raw material to solvent was 1 ： 10.In the condition,the highest extracting content and lowest extract yield of total aconitum alkaloids and paeoniflorin could be obtained.Conclusion The optimized microwave extraction technology could be a potential way with the shorter time and higher extract yield and ME methods is better than the conventional extraction methods.