宝藿苷-Ⅰ对人食管癌细胞Eca-109 Wnt/β-catenin信号转导通路的影响

Effect of baohuoside-Ⅰ on Wnt/β-catenin signaling pathway of human esophageal carcinoma cell Eca-109

目的研究香加皮中提取的宝藿苷-Ⅰ对人食管癌细胞Eca-109的抑制作用及其对Wnt/β-catenin信号转导通路的影响。方法 RT-PCR技术检测不同质量浓度宝藿苷-Ⅰ作用48 h后,细胞β-catenin、Cyclin D1、Survivin m RNA表达的变化,采用流式细胞术分析Eca-109细胞β-catenin、Cyclin D1、Survivin蛋白的表达。结果宝藿苷-I(25、50μg/mL)作用48 h后,Eca-109细胞β-catenin、Cyclin D1、Survivin mRNA及蛋白表达水平明显下降(P<0.01),12.5μg/mL处理组细胞的β-catenin、Cyclin D1、Survivin mRNA及其蛋白表达水平与对照组相比无显著差异。结论宝藿苷-I可能通过下调β-catenin、Cyclin D1、Survivin mRNA及其蛋白表达,影响Wnt/β-catenin信号转导通路,发挥抑制Eca-109细胞增殖作用。

Objective To study the effect of baohuoside-Ⅰ extracted from Periplocae Cortex on proliferation and Wnt/β-catenin signaling pathway of human esophageal carcinoma cell Eca-109.Methods The expressions of β-catenin,Cyclin D1,and Survivin protein in Eca-109 cells were measured with flow cytometry（FCM）.The expressions of β-catenin,Cyclin D1,and Survivin mRNA were detected by RT-PCR.Results After treatment with 25 and 50 μg/mL of baohuoside-Ⅰ for 48 h,the expression levels of β-catenin,Cyclin D1,Survivin mRNA,and protein were decreased significantly（P0.01）,but with 12.5 μg/mL of baohuoside-Ⅰ the expression level was not decreased significantly compared with the control group.Conclusion Baohuoside-Ⅰ from Periplocae Cortex could inhibit the proliferation of Eca-109 cells.This effect associats with down-regulation expression of β-catenin,Cyclin D1,Survivin,and their proteins,which affects on the Wnt/β-catenin signaling pathway.