摘要
目的采用指纹图谱追踪石淋通片工艺过程中黄酮类成分的转移率。方法采用HPLC法分析,色谱柱为Zorbax SB-Aq;流动相为乙腈-0.05%磷酸溶液(梯度洗脱);检测波长为270nm;流速为1mL.min-1;柱温为40℃。结果广金钱草11个主要特征峰的转移率在42.02%～84.17%之间;主要工艺步骤指纹峰的相似度为0.9857～0.9999之间。结论石淋通片的工艺能大部分保留广金钱草黄酮类成分,为石淋通片的工艺评价提供了一定参考。
Objective To study the remaining percentages of flavonoids in the preparation of Tabellae Desmodii Styrocifolii by fingerprint. Methods HPLC was performed on Zorbax SB-Aq column. Acetonitrile -0.05 % phosphoric acid （gradient elution）was used as the mobile phase , detection wavelength was at 270 nm, flow rate was 1 mL·min^-1, and column temperature was 40 ℃. Results The remaining percentages of 11 peaks of Herba desmodii styracifolii were in the range of 42.02 %-84.17 %. The similarity of fingerprint in the technical procedure was 0.985 7-0.999 9. Conclusion Preparation process for TabeUae Desmodii Styracifolii can keep the majority of flavonoids in Herba desmodii styracifolii. The results will provide a reference for the technical estimation of Tabellae Desmodii Styracifolii.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2011年第1期108-110,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省科技计划项目(2009B060700012)
广东省中医药管理局课题(2009256)
关键词
广金钱草
石淋通片
指纹图谱
HPLC
Herba desmodii styracifolii
Tabellae Desmodii Styracifolii
Fingerprint
HPLC
