复方麝香注射液对实验性大鼠视神经挤压伤后视网膜中睫状神经营养因子受体α表达的影响

Effect of Fufang Shexiang Injection on Expression Changes of Ciliary Neurotrophic Factor Receptor ɑ in Retinal after Optic Nerve Injury in Rats

目的:探讨复方麝香注射液对实验性大鼠视神经挤压伤后睫状神经营养因子受体(ciliary neurotrophicfactor receptor,CNTFRα)在视网膜的表达的影响。方法:72只健康SD大鼠,随机分为正常对照组、模型对照组、药物治疗组3组,每组24只。模型对照组和药物治疗组大鼠均采用钳夹法建立视神经挤压伤实验模型,模型成功建立后即予药物治疗组大鼠复方麝香注射液腹腔注射,予正常对照组和模型对照组大鼠等容量的灭菌注射用水腹腔注射,分别于造模后第5、15、30天随机抽取各组8只大鼠处死摘取眼球行免疫组化检测视网膜中CNTFRα。结果:实验发现在正常视网膜中存在CNTFR少量表达;视神经损伤后5、15、30天CNTFRα表达均增高,伤后第5天最高,15天下降,但仍显著高于正常对照组(P<0.05),至30天下降至与正常对照组无显著差异(P>0.05);药物治疗组大鼠视神经损伤后5天CNTFR表达显著高于模型对照组(P<0.05),至伤后治疗15、30天CNTFRα表达极显著高于模型对照组(P<0.01)。结论:视神经损伤后视网膜中CNTFRα表达增加,可能是对视网膜神经节细胞轴突损伤后的自我保护性反应;复方麝香注射液能有效地促进视网膜中CNTFR较长时间高表达。

Objective：To study the effect of Fufang Shexiang injection,compound preparation of Chinese medicine on the expression changes of ciliary neurotrophic factor receptor ɑ（CNTFRɑ）in retinal after optic nerve being crushed in rats.Methods：72 healthy SD rats was divided into three groups：normal group,model group,therapy group.The experimental model of optic nerve with extrusion injury in rats in model group and therapy group was established by crushing optic nerve 3mm retrobulbarly.Immediately after the model was established,the rats in therapy group were given Fufang Shexiang injection by celiac-inject one time a day.And the rats in normal group and model group were given isometrical distilled water by celiac-inject.8 eyeballs were removed respectively to be examined the expression of ciliary neurotrophic factor receptor ɑ in retinal at 5,15 and 30 days after optic nerve injury in each group.The method of immunohistochemistry was applied to measure the changes of CNTFRɑ in retinal.Results：There existed protein expression of CNTFRɑ in the normal retinal.The protein expressions of CNTFRɑ were increased significantly at day 5,15 and 30 after injury,reached peak at day 5 and began to decrease at day 15,But it was still higher than that in the normal group significantly（P0.05）.Then it decreased continuously at day 30 with no significant difference compared with the normal group（P0.05）.In the therapy group,the protein expression of CNTFRɑ was increased significantly at day 5（P0.05）and much significantly at day 15,30（P0.01）.Conclusions：The protein expression increase of CNTFRɑ in the retina after optic nerve injury may be a self-protection response of the retinal neurons.Fufang Shexiang injection can promote and prolong the protein expression of CNTF in the retina after optic nerve injury effectively.