单核细胞及中性粒细胞与内皮细胞粘附的区别

The Difference Between Endothelial-Monocyte and Endothelial-Neutrophil Adhesion

为探讨单核细胞及中性粒细胞与肿瘤坏死因子－ ２ 诱导的内皮细胞粘附的区别，采用髓过氧化酶法测定白细胞与内皮细胞的粘附和抗粘附分子单克隆抗体对其粘附的影响，用酶联免疫法测定内皮细胞粘附分子的表达。结果表明，肿瘤坏死因子－ α呈浓度依赖性地增加内皮细胞与中性粒细胞或单核细胞的粘附，并可诱导内皮细胞上粘附分子的表达增加。肿瘤坏死因子－ α激活内皮细胞后６ 小时，与中性粒细胞的粘附率最高，而激活后２４ 小时，与单核细胞的粘附率最高。抗细胞间粘附分子－ １ 及抗 Ｅ－ 选择素单克隆抗体可显著抑制中性粒细胞与肿瘤坏死因子－ α激活的内皮细胞的粘附，而抗细胞间粘附分子－ １ 及抗血管细胞粘附分子－ １ 单克隆抗体则可抑制单核细胞与肿瘤坏死因子－ α激活的内皮细胞的粘附。结果提示，两种类型的白细胞与肿瘤坏死因子－ α诱导的内皮细胞的粘附具有不同的特点。

Aim To study the difference between endothelial-monocyte and endothelial-neutrophil adhesion. Methods Cell adhesion was measured by testing leukocyte myeloperoxidase activity, the cell adhesion was blocked by monoclonal antibodies to ICAM-1, E-selectin and VCAM-1, the expression of adhesion molecules was measured by ELISA. Results TNF-α (100～1 000 ku/L)increased the adhesion of EC to neutrophils or monocytes concentration-dependently. The increased expression of ICAM-1, E-selectin and VCAM-1 was contributed to TNF-α induced upregulation of leukocyte-endothelial adhesion. The neutrophil-endothelial adhesion was peaked at 6 h after TNF-α stimulation of endothelial, while monocyte-endothelial adhesion was peaked at 24 h. The monoclonal antibodies to ICAM-1 and E-selectin significantly inhibited TNF-α induced endothelial-neutrophil adhesion, nevertheless, the antibody to VCAM-1 had no effect on endothelial-neutrophil adhesion. On the other hand, the antibodies to ICAM-1 or VCAM-1 but not the antibody to E-selectin inhibited TNF-α induced endothelial-monocyte adhesion. Conclusion Neutrophil and monocyte showed different characteristics in adhesion to TNF-α induced endothelial cells.