COX-2对结肠癌细胞CCR7表达的调节

Regulation the CCR7 expression in tumor cells by COX-2

目的探讨COX-2对肿瘤细胞CCR7表达的调节及其机制。方法用NS-398、PGE2、Con A及EP受体激动/抑制剂作用于肿瘤细胞SW620,通过RT-PCR及Western印迹检测其对肿瘤细胞CCR7表达的影响。趋化小室研究NS-398、PGE2及Con A等化合物作用于SW620细胞后,对CCL21刺激引起的趋化、侵袭细胞的影响。结果 NS-398、AH-23848能抑制SW620细胞CCR7表达,抑制CCL21刺激趋化、侵袭能力,而PGE2、Con A的作用则与其相反。SC-19220、17-phenyl trinor PGE2及AH-6809阻断或激动其他EP受体后,没有观察到此变化。结论在SW620细胞,抑制COX-2和EP4受体能下调CCR7的功能性表达,为进一步研究COX-2、CCR7促进肿瘤转移的机制提供理论基础,也提示COX-2与CCR7表达在肿瘤转移中有协同作用,在肿瘤干预治疗中具有潜在的应用价值。

[ Objective ] To explore the regulation of CCR7 expression by COX-2 and its mechanism. [Methods ] The expression of CCR7 was detected by RT-PCR and Western blot in SW620 cells, which incubated with NS-398 PGE2.Con A.EP receptor agonists or antagonist, then the effect of ehemotaetie and invasive responses were investigated after the treated tumor cells stimulated by CCL21, through ehemotactic chamber. [Results] In SW620 cells could be found the expressed of CCR7, ehemotactic and invasive responses activated by CCL21 were significantly inhibited when treated with NS-398 and AH-23848. The negative results could be detected when treated with PGE2 and Con A. There were not significant differences when .treated with SC-19220, 17-phenyl trinor PGE2, AH-6809. [ Conclusion ] In SW620 cells, the inhibitors of COX-2 and EP4 receptor can down-regulate the functional expression of CCR7, could be helpful to further studying the mechanism of COX-2 and CCR7 in promoting tumour metastasis, and indicate the synergie effect of COX-2 and CCR7 in tumour metastasis and the potential role in cancer therapy.