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2型糖尿病患者PBMC组蛋白甲基化研究 被引量：2

Research on histone methylation in PBMC of type 2 diabetic patients
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摘要 目的该研究探讨了2型糖尿病(T2DM)患者外周血单个核细胞(PBMC)组蛋白修饰情况。方法收集12例T2DM患者及12例健康对照者的PBMC,采用H3K4/H3K9甲基化检测试剂盒提取组蛋白和检测H3K4/H3K9甲基化水平。实时荧光定量PCR法检测T2DM患者的PBMC组蛋白甲基转移酶和组蛋白去甲基化酶基因的mRNA表达水平。结果与健康对照组比较,T2DM患者的PBMC组蛋白H3K4甲基化水平升高。实时定量PCR结果显示T2DM患者组PBMC的SET1、SUV39H1、SUV39H2、KDM3A、KDM5B和LSD1基因表达分别是健康对照组的3.06、0.46、0.48、0.43、3.07和0.35倍。结论 2型糖尿病患者外周血PBMC组蛋白甲基化呈现异常。 [ Objective ] To study file histone methylafion patterns in peripheral blood mononuelear cells （PBMC） from type 2 diabetic patients. [Methods] PBMC from 12 type 2 diabetic patients and 12 healthy controls were recruited. The global H3K4/H3K9 methylation was determined by assay kit. The differential expression of the histone methyltransferases and histone demethylases were measured by real-time polymerase chain reaction （RT-PCR）. [Results] Increased global H3K4 methylation was observed in PBMC of type 2 diabetic patients compared with controls. The mRNA level of gene SET1, SUV39H1, SUV39H2, KDM3A, KDM5B, LSD1 in patients＇ PBMC compared with healthy controls was 3.06, 0.46, 0.48, 0.43, 3.07, 0.35 fold respectively. [Conclusion] Histone methylation appears abnormal in PBMC of Type 2 diabetic patients.
作者 侯粲 李一君 蔺怡 吴碧琛 周智广
机构地区 中南大学湘雅二医院内分泌科(代谢内分泌研究所糖尿病免疫学教育部重点实验室)
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2011年第1期44-47,共4页 China Journal of Modern Medicine
基金 湖南省高校科技创新团队支持计划资助[No:湘教通(2008)244号]
关键词 2型糖尿病 外周血单个核细胞 组蛋白甲基化 type 2 diabetes mellitus peripheral blood mononuelear cells histone methylation
分类号 R587.1 [医药卫生—内分泌]
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参考文献11

  • 1PIN NEY SE, SIMMONS RA. Epigenetic mechanisms in the development of type 2 diabetes [J]. Trends Endocrinol Metab, 2010, 21(4): 223-229.
  • 2侯粲,李一君,周智广.糖尿病表观遗传学研究进展[J].中华医学杂志,2009,89(10):715-717. 被引量：6
  • 3SIMS RJ, REINBERG D. Is there a code embedded in proteins that is based on post-translational modifications [J]. Nat Rev Mol Cell Biol, 2008, 9(10): 815-820.
  • 4EGGER G, HANG G, APARICIO A, et al. Epigenetics in human disease and prospects for epigenetic therapy [J]. Nature, 2004, 429(6990): 457--463.
  • 5INAGAKI T, TACHIBANA M, MAGOORI K, et al. Obesity and metabolic syndrome in histone demethylase JHDM2a-deficient mice[J]. Genes Cells, 2009, 14: 991-1001.
  • 6CHAKRABARTI SK, FRANCIS J, ZIESMANN SM, et al. Covalent histone modifications underlie the developmental regulation of insulin gene transcription in pancreatic β cells[J]. J Biol Chem, 2003, 278: 23617-23623.
  • 7MUTSKOV V, RAAKA BM., FELSENFELD G, et al. The human insulingene displays transcriptionally active epigenetie marks in islet-derived mesenchymal precursor ceils in the absence of insulin expression[J]. Stem Cells, 2007, 25: 3223-3233.
  • 8MIAO F, WU XW, ZHANG LX, et al. Genome-wide analysis of histone lysine methylation variations caused by diabetic conditions in human monocyte[J]. J Biol Chem, 2007, 282: 13854-13863.
  • 9MIAO F, IRENE GG, LINDA L, et al. In vivo chromatin remodeling events leading to inflammatory gene transcription under diabetic conditions [J]. J Biol Chem, 2004, 279: 18091-18097.
  • 10BRASACCHIO D, OKABE J, TIKELLIS C, et al. Hyperglycemia induces a dynamic cooperativity of histone methylase and demethylase enzymes associated with gene-activating epigenetic marks that coexist on the lysine tail [J]. Diabetes. 2009, 58:1229-1236.

二级参考文献22

  • 1Cave H, Polak M, Drunat S, et al. Refinement of the 6q chromosomal region implicated in transient neonatal diabetes. Diabetes, 2000, 49: 108-113.
  • 2Temple IK, Shield JP. Transient neonatal diabetes: a disorder of imprinting. J Med Genet, 2002, 39: 872-875.
  • 3Arima T, Drewell RA, Arney KL, et al. A conserved imprinting control region at the HYMAI/ZAC domain is implicated in transient neonatal diabetes mellitus. Hum Mol Genet, 2001, 10: 1475 -1483.
  • 4Mackay DJ, Coupe AM, Shield JP, et al. Relaxation of imprinted expression of ZAC and HYMAI in a patient with transient neonatal diabetes mellitus. Hum Genet, 2002, 110: 139-144.
  • 5Julier C, Hyer RN, Davies J, et al. Insulin-IGF2 region on chromosome llp encodes a gene implicated in HLA-DR4- dependent diabetes susceptibility. Nature, 1991, 354: 155-159.
  • 6Polychronakos C, Kukuvitis A. Parental genomic imprinting in endocrinopathies. Eur J Endocrinol, 2002, 147: 561-569.
  • 7McCann JA, Xu YQ, Frechette R, et al. The insulin-like growth factor-II receptor gene is associated with type 1 diabetes: evidence of a maternal effect. J Clin Endocrinol Metab, 2004, 89: 5700- 5706.
  • 8Wu W, Shang J, Feng Y, et al. Identification of glucose- dependant insulin secretion targets in pancreatic beta cells by combining defined-mechanism compound library screening and siRNA gene silencing. J Biomol Screen, 2008, 13 : 128-134.
  • 9Marzban L, Tomas A, Becker TC, et al. Small interfering RNA- mediated suppression of proislet amyloid polypeptide expression inhibits islet amyloid formation and enhances survival of human islets in culture. Diabetes, 2008, 57 : 3045-3055.
  • 10Poy MN, Eliasson L, Krutzfeldt J, et al . A pancreatic isletspecific microRNA regulates insulin secretion. Nature, 2004, 432 : 226-230.

共引文献5

同被引文献72

  • 1刘飞,靳飞,翟晓巧,贾峰.组蛋白甲基化和去甲基化研究进展[J].中国农学通报,2007,23(2):56-59. 被引量：4
  • 2张垚,李林鲜,李悦恒,李惠.胰岛素受体底物家族与Ⅱ型糖尿病关系性的研究进展[J].现代生物医学进展,2007,7(2):312-315. 被引量：9
  • 3Mccarthy MI. Genomics, type 2 diabetes, and obesity [ J]. N Engl J Med, 2010,363 (24) :2339-2350.
  • 4Ling C, Del GS, Lupi R, et al. Epigenetic regulation of PPARGC1A in human type 2 diabetic islets and effect on insulin secretion [ J ]. Diabetologia, 2008,51 (4) :615-622.
  • 5Ling C, Groop L. Epigenetics : a molecular link between environmental factors and type 2 diabetes[J]. Diabetes, 2009,58(12) :2718-2725.
  • 6Liu L, Li Y, Tollefsbol TO. Gene-environment interactions and epigenetie basis of human diseases[ Jl. Curr Issues Mol Biol, 2008,10 (1-2) :25-36.
  • 7Waddington CH. The epigenotype [ J ]. Int J Epidemiology, 2012,41 (1) :10-13.
  • 8Bender J. DNA methylation and epigenetics [ J ]. Annu Rev Plant Biol, 2004,55:41-68.
  • 9Beck S, Rakyan VK. The methylome: approaches for global DNA methylation profiling[ J]. Trends Genet, 2008,24(5 ) :231-237.
  • 10Albert M, Helin K. Histone methyltransferases in cancer [ J ]. Semin Cell Dev Biol, 2010,21 (2) :209-220.

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二级引证文献5

中国现代医学杂志
2011年 第1期

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