Caspase-3与Caspase-6蛋白在肾透明细胞癌中的表达及意义

Expression and significance of Caspase-3 and Caspase-6 proteins in RCCC

目的检测Caspase-3、Caspase-6蛋白在肾透明细胞癌(RCCC)、癌旁、正常肾组织中的表达,探讨两者在不同临床分期、病理分级的表达及与预后的关系,并分析两者的相关性。方法采用免疫组化的方法检测75例RCCC及其癌旁组织、15例正常肾组织标本中的Caspase-3和Caspase-6蛋白表达水平。结果 Caspase-3和Caspase-6蛋白在RCCC中的阳性表达率(74.67%,76.00%)低于癌旁组织(92.00%,90.67%)和正常肾组织(93.33%,86.67%),差异有统计学意义(P<0.05)。Caspase-3病理G3期阳性表达率(53.58%)低于G1期(87.50%),P<0.05;临床Ⅲ、Ⅳ期阳性表达率(63.64%)低于Ⅰ期(87.97%),P<0.05;淋巴结转移阳性组中阳性表达率(55.56%)低于阴性组(80.70%)。Caspase-6在不同病理分级和临床分期以及是否有淋巴结转移中阳性表率无显著性差异。两者在RCCC中的阳性表达率无相关性(C=0.0921,P>0.05)。结论 Caspase-3和Cas-pase-6表达减少导致细胞凋亡减少、与肾细胞癌发病有密切关系,但不是肾细胞癌有价值的标记物。且两者的表达降低并不存在相关性。

[ Objective ] To investigate the expression of caspase-3 and caspase-6 proteins in human renal clear cell carcinoma （RCCC）, para-tumor tissue and normal renal tissue. To probe into the expression of caspase-3 and easpase-6 proteins were correlated with the clinical pathologiea characters and prognosis of RCCC and analyze the relation of them. [Methods] 75 RCCC and their para-tumor and 15 normal renal tissue were analyzed for expression of easpase-3 and easpase-6 proteins by SP immunohischemistry and correlation with the characters and prognosis of RCCC. [Results ] The positive rate of caspase-3 and caspase-6 proteins in all RCCC（74.67%, 76.00%） lower than that in para-tumor（92.00%,90.67%） and normal renal tissue（93.33%,86.67%）, （P 〈0.05）. The difference of caspase-3 between G3（53.58%） and G1（87.50%）, Ⅲ-Ⅳ（63.64%） and Ⅰ （87.97%） were significantly（P 〈0.05）. The positive rate and negative rate of caspase-3 protein in that with lymph nodes metastasis were 55.56% and 80.70%, the difference between them was significantly （P 〈0.05）. The difference of caspase-6＇ positive rate in all clinical stage, pathologic grade, whether or not lymph nodes metastasis were not significantly .In RCCC, the lower positive expressions of easpase-3 and caspase-6 proteins were independence from each other （C=0.0921, P 〉0.05）. [ Conclusions ] Abnormality decreased expression of easpase-3 and easpase-6 were involved in development and progression of renal clear cell carcinoma by decreasing apoptosis., but they were not valuable marker for tumor biological behavior and there were not necessarily correlation between them in the lower positive expressions.