支气管肺泡灌洗液hnRNPA2/B1、NSE和CEA联合检测在肺癌患者中的临床意义

Clinical value of determinating hnRNPA2/B1 NSE and CEA in bronchoalveolar lavage fluid of lung cancer patients

目的探讨支气管肺泡灌洗液(BALF)中异质性胞核核糖核蛋白A2/B1(hn RNPA2/B1)、神经元烯醇化酶(NSE)和癌胚抗原(CEA)水平测定在肺癌患者中的临床意义。方法对该院2006年1月～2009年6月之间88例肺癌病人(实验组)及68例肺良性疾病患者(对照组)行BALF中hn RNPA2/B1、NES和CEA的水平测定。结果肺癌组BALF中hn RNPA2/B1、NES和CEA明显高于肺良性疾病组(hnRNPA2/B1:(78.82±4.50)vs(32.43±4.73),P=0.0001;NSE:(28.92±2.84)vs(11.66±1.58),P=0.0008;CEA:(54.82±5.50)vs15.84±2.63,P=0.0002),差异有统计学意义(P<0.05),三项联合检测可明显提高肺癌阳性检出率(P<0.05)。hnRNPA2/B1及CEA非小细胞癌明显高于小细胞癌(P<0.05),NSE在小细胞癌中明显高于非小细胞癌(P<0.05),NSE在小细胞癌中明显高于非小细胞癌(P<0.05)。结论 hnRNPA2/B1、NSE和CEA与肺癌的病情变化呈负相关,联合应用可提高其敏感性,尤其在支气管肺泡灌洗液中更为敏感,对肺癌患者早期诊断、病情动态变化、判断预后有一定的参考价值。

[Objective] To explore the clinical value of determinating hnRNPA2/B1, NSE and CEA in bronchoalveolar lavage fluid （BALF） on lung cancer patients. [Methods] HnRNPA2/B1, NSE and CEA in BALF were detected of 88 lung cancer patients （experimental group） and 68 patients of benign lung disease （control group）. [Results] The concentration level of hnRNPA2/B1, NSE and CEA in experimental group was signifieandy higher than that in contrast group （P 〈0.05） （hn RNPA2/BI： 78.82±4.50 vs 32.43±4.73, P =0.0001; NSE： 28.92±2.84 vs 11.66±1.58, P =0.0008; CEA： 54.82±5.50 vs 15.84±2.63, P =0.0002） And combined detection has highest positive rate on 88 lung cancer patients. The concentration level of hnRNPA2/B1 and CEA in non-small-cell lung cancer group was significantly higher than that in small-eell lung cancer group （P 〈0.05）. The concentration level of NSE in small-cell lung cancer group was significandy higher than that in non-small-cell lung cancer group （P 〈0.05）. [Conclusion] The levels of hnRNPA2/B1, NSE and CEA were negatively correlated with lung cancer patient＇s condition, and combined detection can improve positive rate,especially in BALF. Combined detection hnRNPA2/B1, NSE and CEA in bronchoalveolar lavage fluid on lung cancer patients could be applied to reflect the patient＇s condition and assess prognosis.