高菜游离小孢子培养与植株再生

Isolated microspore culture and plant regeneration in Brassica juncea Czern. et Coss.

以3个高菜品种三池高菜、紫高菜、柳川大缩缅为试材,研究不同培养条件对小孢子胚诱导和植株再生的影响。结果表明：基因型是限制小孢子胚发生的主要因素,相同处理条件下,不同基因型之间出胚率有显著差异,紫高菜出胚率为16.00%,三池高菜出胚率为10.67%,而柳川大缩缅未产生小孢子胚;热激诱导时间对胚发生也有明显影响,当热激（33℃）时间为48-72 h时出胚率有较大提高;转胚后适宜胚状体生芽培养基为B5＋0.02 mg.L-1TDZ＋0.02 mg.L-1NAA＋0.05 mg.L-1KT＋1 g.L-1活性炭＋30 g.L-1蔗糖＋8 g.L-1琼脂,生芽率为88%;继代生根培养基以1/2MS＋0.2 mg.L-1NAA＋30 g.L-1蔗糖＋8 g.L-1琼脂为宜,紫高菜生根率为100.00%,三池高菜生根率为96.00%。

Different culture conditions affecting microspore-derived embryos and plant regeneration were studied by using 3 different varieties of Brassica juncea Czern.et Coss..The results indicated that the genotype was the key factor of limiting microspore embryogenesis,and that with the same treated condition,the rates of microspore embryos were significant by different between genotypes：the embryo frequency of Zi,16.00%;Sanchi,10.67%,and Liuchuandasuomian,none.Heat-shock inducted time also produced obvious influence on microspore embryogenesis,the treatment（33 ℃）for 48-72 hours promoted the rate of embryoid induction.With the suitable medium for shooting being B5＋0.02 mg·L-1 TDZ＋0.02 mg·L-1 NAA＋0.05 mg·L-1 KT＋1 g·L-1 active carbon＋30 g·L-1 sugar＋8 g·L-1 agarose,the shoot induction rate came up to 88%.The root induction rate of Zi was 100.00%,and Sanchi was 96.00% when the medium was 1/2MS＋0.2 mg·L-1 NAA＋30 g·L-1 sugar＋8 g·L-1 agarose.