摘要
为了探明东毕吸虫成虫可溶性抗原的多肽组分,供今后在免疫诊断和防治东毕吸虫的应用,本试验应用SDS- PAGE首次对绒山羊体内寄生的土耳其斯坦东毕吸虫成虫可溶性抗原的多肽组分进行了分析。结果表明该抗原有17 条多肽带,其分子量范围25~93KD.其中主带7 条,分别为29、30、38、40、67、78、91KD。本试验初步阐明了土耳其斯坦东毕吸虫成虫抗原的多肽组分,为进一步对该病免疫诊断用抗原的分离、纯化。
To verify the polypeptide components of Ornithobilharzia turkestanicum adult antigens and be applied in the immunodiagnosis and immunization of Ornithobilharziasis,soluble adult antigen of the O.turkestanicum ,which was collected from Fine fair goat,was analysed by sodium docdecyl sulface polyacrylamide gel electrophoresis (SDS PAGE) in the first time.The result indicates that O.turkestanicum adult antigen has 17 polypeptide bands with MW ranging from 29 to 93KD.The main seven bands are 39,30,38,40,67,78 and 91KD.The present study expounds the polypeptide components of O.turkestaicum and made the base of further separation and purification in studies on immunodiagnosis antigens.
出处
《中国兽医寄生虫病》
1999年第4期3-4,共2页
Chinese Journal of Veterinary Parasitology
基金
黑龙江省"九五"科技攻关项目