摘要
目的建立液质联用（LC-MS）直接沉淀法测定人血浆匹伐他汀的浓度。方法 Waters Cosmosil Packed Coulmn C18-MS-Ⅱ（2.0mm×150mm,5μm）色谱柱,流动相为10mmol·L-1甲酸胺（含0.1%甲酸）-乙腈（40∶60）,流速为0.3mL·min-1,进样体积为20μL,柱温为40℃,样品室温度为15℃,采用内标定量法,内标为罗素伐他汀。结果匹伐他汀线性范围为0.5~1000 ng·mL-1,最低检测限为0.1ng·mL-1,方法灵敏、稳定、特异性高,并已成功地应用到人血浆匹伐他汀药动学研究。结论该方法简便、准确、重复性好,可以准确地定量人血浆匹伐他汀的浓度,适于药理科研。
OBJECTIVE To develop a LC-MS method with direct precipitation of sample protein to determinate the concentration of pitavastatin in human plasma.METHODS LC-MS equipment with ESI source and Waters Cosmosil Packed Coulmn C18-MS-Ⅱ（2.0 mm×150 mm,5 μm） were used in the experiment.The column temperature was set at 40 ℃.10 mmol·L-1 ammonium formate（contain 0.1% formic acid）-acetonitrile（40∶60） was used as mobile phase and the flow rate was 0.3 mL·min-1.The sample room temperature was set at 15 ℃ and the injection volume was 20 μL.Rosuvastatin was taken as the internal standard.RESULTS Pitavastatin was linear in the range of 0.5-1 000 ng·mL-1.The limitation of detection for pitavastatin was 0.1 ng·mL-1.The method was sensitivity,stability and specificity and was used for pharmacokinetic study of pitavastatin in human successfully.CONCLUSION The method is simple,accurate,reproducibility for the determination of pitavastatin in human plasma and suitable for the study in pharmacology.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第2期134-137,共4页
Chinese Pharmaceutical Journal
