木屑栽培香菇胞外纤维素酶和磷酸二酯酶的活性变化

Activity Variation of Extracellular Cellulase and Phosphodiesterase of Lentinula edodes Cultured in Sawdust Media

不同木屑培养料栽培香菇菌丝生长盛期、原基形成期和子实体成熟期胞外Cx酶(羧甲基纤维素酶)和C_2酶(β-葡萄糖苷酶)的活性是逐渐增强的,以确保碳素营养的充分供应。不同木屑培养料的成分只影响酶活性的相对大小,而不影响酶活性的变化规律。纤维素酶活性对子实体形成不起决定作用。磷酸二酯酶活性先渐增,至菇蕾形成才急剧下降,抑制其活性是诱导香菇子实体和产量形成的关键因素。

The activity variation of extracellular cellulase (Cx and C2 - enzyme) and phosphodiesterase in the extraction of L. edodes hyphae cultured in sawdust media during the full spawn - run stage, primordium formation stage and fruitbody maturity stage was studied. The activity of extracellular cellulase gradually increased with the growth of hyphae to ensure sufficient C nutrition supply. It was proved in our test that the different sawdust medium could only affect the relative enzyme activity, but not the variation law of enzyme activity, that the activity of extracellular cellulase did not play the determinant effect on the formation of the fruitbody, that the phosphodiesterase activity increased gradually at first, then it reduced sharply during the primordium formation stage, and that the inhibition of phosphodiesterase activity during the primordium formation stage might be positively correlated with the yield of L. edodes, and the active ingredient produced by phosphodiesterase was the key factor in inducing the fruitbody formation and the yield of L. edodes.