羊肚菌多糖MEP-SP1分离纯化及性质鉴定

Isolation, Purification and Property Identification of MEP-SP1

羊肚菌「Ｍｏｒｃｈｅｌｌａ ｅｓｃｕｌｅｎｔａ （Ｌ．）；Ｐｅｒｓ．」多糖具有降血脂及抗肿瘤功能。从羊肚菌培养液中得到分量１００００－１０００００万的多糖ＭＥＰ－ＳＰ，采用ＤＥＡＥ－纤维素离柱及Ｓｅｐｈａｒｏｓｅ ＣＬ－６Ｂ柱，以水为洗脱液，分离得水溶性多糖ＭＥＰ－ＳＰ１。实验结果表明，ＭＥＰ－ＳＰ１是一种由木糖，葡萄糖，阿拉伯糖和半乳糖残基为重复单元组成的杂多糖，分子量约为１１５００。

The polysaccharide in Morchella esculenta (L. ) Pers. (MEP) has the most effective blood-fat-lowering and anticarcinogenic effect. The polysaccharide MEP-SP with the molecular weight of 10 000-100 000 was obtained from Morchella esculenta culture solution. And the water-soluble polysacchride MEP-SP1 was purified and separated by DEAE-ion exchange cellulose and Sepharose CL-6B using water as eluant. The experiment result showed that MEP-SP1 was a kind of heteropolysaccharide composed of xylose, glucose, arabinose und galactose at a moleratio of 29:24:61:39. Its molecular weight was 11 500. IR and 1HNMR analysis showed that its main chains were composed of β-pyranglycoside linkage.