RP-HPLC法测定替米沙坦血浆药物质量浓度

Determination of telmisartan in human plasma by RP-HPLC

目的建立人血清中替米沙坦的RP-HPLC测定法。方法以厄贝沙坦为内标,采用Agilent Zorbox C18柱（250 mm×4.6mm,5μm）;流动相：乙腈-水（1 mL.L-1三乙胺,用磷酸调节pH至3.08）（52∶48）;流速：1.0 mL.min^-1;柱温：25℃;激发波长300 nm,发射波长380 nm。结果替米沙坦与内标峰形良好,分离完全,保留时间分别为4.8和5.2 min,日内和日间RSD均小于10%,替米沙坦质量浓度在2~1 000μg.L-1范围内线性关系良好,r=0.999 9。结论该法操作简便,结果可靠,适用于替米沙坦血浆药物质量浓度的测定。

Objective To establish an assay method for telmisartan in human plasma with reversed-phase high performance liquid chromatography.Methods The internal standard was irbesartan,and then the samples were separated on an Agilent Zorbox-C18 column using acetonitrile-water（pH was adjusted to 3.08 by phosphoric acid）（52∶48） as mobile phase at a flow rate of 1.0 mL·min-1.The column temperature was set at 25 ℃.Telmisartan and irbesartan were detected by fluorescene detection（Ex=300 nm,Em=380 nm）.Results Telmisartan and irbesartan were separated well.The linear ranges of telmisartan was 2-1 000 μg·L-1（r=0.999 9）.The intra-day RSD and inter-day RSD telmisartan was less than 10%.Conclusion The method is simple,rapid,sensitive and accurate,and it is applicable for therapeutic drug monitoring of telmisartan in human plasma.