大白鼠肝脏醋酸局部注射的实验研究

AN EXPERIMENTAL STUDY ON INTRAHEPATIC LOCAL INJECTION OF ACETIC ACID RATS

目的 探讨醋酸局部注射对肝组织的影响 ,探讨该法治疗肝癌的机制并与无水乙醇治疗进行比较。方法 SD大鼠 80只 ,随机分为A、B、C、D 4组 (n =2 0 )。分别将 10 0 μl的醋酸液、无水乙醇液分别注入同一大鼠肝外叶、左外侧叶。A、B、C、D组分别在注射后 1h、2d、4d及 6d后剖杀 ,观察肝脏病变。结果 醋酸组在坏死灶直径、坏死率、病灶内及边缘区出血、囊腔形成及网络样损伤等方面均较无水乙醇组有显著差异。结论 醋酸具有比无水乙醇更显著的肝细胞损伤作用。

Objective To illustrate the mechanism of local acetic acid injection as a therapy (PAIT) for liver cancer by comparing the effects on liver tissue produced by local acetic acid injection with that by dehydrated alcohol.Methods 100 μl of 40% acetic acid and the same amount of dehydrated alcohol (ethanol) were separately injected into different lobes of the liver in 80 rats.The animal were randomly divided into A,B,C and D groups (n=20 each),which were killed 1 h,2 d,4 d and 6 d after injection respectively,to study the liver pathology.Results Acetic acid was found to be superior to dehydrated alcohol,as indicated by less bleeding in and around the lesion,more marked necrosis in size and frequency,more cystic formation and network form damage (P<0.01～P<0.05). Conclusion Having better destructive actions on liver tissue than dehydrated alcohol,acetic acid administer as PAIT should be a rewarding treatment for liver cancer.