转DREB3基因抗旱大豆对土壤微生物群落及有益微生物的影响

Effect of transgenic drought resistant soybean on soil microbial community and beneficial microorganism

采用盆栽试验方法,在正常水分管理和干旱胁迫条件下研究了转DREB3基因抗旱大豆对土壤细菌、放线菌、真菌及两种有益菌木霉菌和自生固氮菌数量的影响。结果表明,在正常水分管理条件下,只有VE期转基因抗旱大豆根际土壤中细菌数量显著增多,放线菌数量显著减少。干旱胁迫下,转基因抗旱大豆根际土壤中放线菌数量也只在R1期与正常水分管理相比显著减少。转基因大豆在正常水分管理下的R1、R4期和干旱胁迫下的VE、R4期,对根际土壤细菌、放线菌、真菌、木霉菌和自生固氮菌数量无显著影响。

Pot culture method was used to study the effect of transgenic drought resistant soybean （TDRS） and no transgenic drought resistant soybean （NTDRS） on soil bacteria number, actinomycetes number, fungi number and two kinds of the beneficial microorganisms named trichoderma and azoto- bacter. The results showed that the soil bacteria number was significant increased and actinomycetes number decreased in rhJzosphere of TDRS only at VE growth stage under normal soil water condition. Under water stress condition actinomycetes number was significant decreased only at R1 growth stage in rhizosphere of TDRS compared with that of in the normal water condition. There was no significant effect on number of bacteria, actinomycetes, fungi, trichoderma and azotobacter at R1, R4 under normal water condition and R1, R4 under water stress condition in rhizosphere of TDRS.