RP-HPLC测定柴胡滴丸中柴胡皂苷a、d的含量

Determination of Saikosaponin a,d content in Chaihu dripping pills by RP-HPLC

目的采用反相高效液相色谱法（RP—HPLC）对柴胡滴丸中柴胡皂苷a、d含量进行测定。方法色谱柱为Agilent EclipseXDB-C18（4．6mm×250mm。5μm），以乙腈和水为流动相，采用梯度洗脱，检测波长为210nm。结果柴胡皂苷a在0．49～7．84μg（R=1）范围内、柴胡皂苷d在0．0778～1．2448μg（R=1）范围内，浓度与峰面积呈良好线性关系，方法平均回收率分别为98．7％（RSD=1．5％）、99．2％（RSD=1．6％）。结论该方法对柴胡滴丸中柴胡皂苷a、d含量的测定简便易行，结果准确可靠，适用于对柴胡滴丸进行质量控制。

OBJECTIVE The content of saikosaponina and saikosaponin d in Chaihu dripping pills had been determined by RP-HPLC method. METHODS The chromatographic column was Agilent Eclipse XDB-CI8 （4.6mm × 250mm, 5μm ）. Mobile phase is acetonitrile and water solution （gradient elution）. The detection wave length was 210nm. RESULTS The good linear detection wncentration range of saikosaponin was0.49-7.84μg （R= 1）and saikosaponin d was 0.07？8-- 1.2448μg（R=1）.The recovery of saikosaponin a was 98.7% （RSD= 1.5 % ） and saikosaponin d was 99.2 % （RSD = 1.6 % ）. CONCLUSION The present method for determining con tent of saikosaponin a and saikosaponin d in Chaihu dripping pills is simple, feasible with accurate and reliable resuits, which is suitable for the quality control of this preparation.