摘要
建立了用气相色谱法测定麝香酮血药浓 度和脑药 浓度的方法。 Ｓ Ｅ－ ５４ 石英毛 细管柱 （０ ．２５ ｍ ｍ ×３０ ｍ ） ， Ｆ Ｉ Ｄ 检测器， 以高纯氮为载气， 异补骨脂素为内标， 进行定量测 定。 最低检测限为 ０ ．５ ｎｇ ， 麝香酮血药浓度在 ７０ ｎｇ／ ｍ Ｌ～４４８０ ｎｇ／ ｍ Ｌ 范围内线性关系良好 （ｒ ＝ ０ ．９９９９） ， 脑药浓度在 ７０ ｎｇ／ｇ ～４４８０ ｎｇ／ｇ 范围内线性关系良好 （ｒ ＝ ０ ．９９９７） ， 回收率 （ Ｒ Ｓ Ｄ） 血浆中为８８ ％ ， 脑组织中为 ８０ ％ 。该法重现性好， 特异性强。
The method for the separation quantitative determination of muscone in plasma and brain (using isopsoralen as the internal standard)by gas chromatography was established.3% SE-54 capillary column in conjunction with FID was used as the detector and high purity N 2 as the carrier gas.The minimum detectable quantity of muscone was 0.5ng.The linear range of muscone was at 70 ng/mL～4480 ng/mL in plasma(r= 0.9999) and 70 ng/g～4480 ng/g in brain(r= 0.9997). The average recovery rate was 88% in plasma and 80% in brain.This method has a high reproducibility and specifity.
出处
《中药新药与临床药理》
CAS
CSCD
1999年第5期276-278,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
