丁胱亚磺酰亚胺对A cc 2细胞的放射增敏效应研究

Studies of radiosensitizative effect of BSO on Acc 2 cells

目的 研究新型放射增敏剂丁胱亚磺酰亚胺（ｂｕｔｈｉｏｎｅ ｓｕｌｆｏｘｉｍ ｉｎ， Ｂ Ｓ Ｏ）对 Ａｃｃ２ 细胞的放射增敏效应。方法 采用克隆形成法，首先观察了 Ｂ Ｓ Ｏ 在乏氧状态和有氧状态下对 Ａｃｃ２ 细胞的药物毒性，然后研究了 Ｂ Ｓ Ｏ 在乏氧和有氧状态下对 Ａｃｃ２ 细胞的放射增敏效应。结果 Ｂ Ｓ Ｏ 在乏氧状态和有氧状态时，其半数抑制剂量分别为８０μｍ ｏｌ／ Ｌ、１２０μｍ ｏｌ／ Ｌ。当 Ｂ Ｓ Ｏ 在 Ｉ Ｄ２０、 Ｉ Ｄ１０ 的药物剂量时，在乏氧状态下，其 Ｓ Ｅ Ｒ 值分别为１５２１ 和１２６３，在有氧状态下其 Ｓ Ｅ Ｒ 值分别为１２９６ 和１１５３。结论 Ｂ Ｓ Ｏ 对 Ａｃｃ２ 细胞有放射增敏作用， Ｂ Ｓ Ｏ 在乏氧状态下的增敏效应比有氧状态下的放射增敏效应强。

Objective To study the radiosensitizative effect of buthione sulfoximin (BSO) on Acc 2 cells.Methods First,the pharmotoxicity of BSO to Acc 2 cells under hypoxic or aerobic conditions was measured.Second,the radiosensitizative effect of BSO on Acc 2 cells under hypoxic or aerobic conditions was studied.Plating efficiency assay was used in this experiment.Results The 50% inhibition dose (ID50) of BSO to Acc 2 cells under hypoxic or aerobic condition was 80 μmol/L、120μmol/L,respectively.When the dose of BSO was ID20 and ID10 under hypoxic or aerobic condition the SER was 1.521、1.263 and 1.296、1.153, respectively.Conclusion BSO had radiosensitizative effect on Acc 2 cells both under hypoxic or aerobic condition.The radiosensitizative effect of BSO on Acc 2 cells under hypoxic condition was stronger than that under aerobic condition.