摘要
采用高效液相色谱法,以C18色谱柱为分析柱,乙腈-0.2%磷酸溶液（7∶93）为流动相,检测波长273 nm,柱温25℃,并利用超声微波协同萃取法对没食子酸的提取进行单因素的考察,建立超声微波协同萃取-HPLC法测定五倍子中没食子酸含量。没食子酸进样量在1~5μg范围内峰面积与其浓度有良好的线性关系（r=0.999 7）,平均回收率95.62%,RSD=1.58%（n=5）,超声微波协同萃取的适宜条件：微波功率230 W,超声功率50 W,提取时间810 s,料液比1∶30。此方法简便、快速、准确,可用于没食子酸的提取和定性定量分析。
The determination of gallic acid in galla chinensis by ultrasonic wave and microwave synergic extraction-HPLC was set up.The condition of HPLC was as follows： AQCl8（150 mm×4.6 mm,5 μm）column,acetonitrile-0.2%phosphoric aid（7∶ 93） as mobile phase,with a detection wavelength of 273 nm and the temperature to 25℃.The effects of factors on the extraction of gallic acid was examined through single factor experiment.Results showed that the linear relationship was good in the range of 1~5 μg for gallic acid.The recovery rate was 95.62% with RSD=1.58%（n=5）.The suitable conditions was microwave power of 230W,ultrasonic wave power of 50W,the time of 810s and the liquid-solid ratio of 1/30.The method was convenient,rapid and accurate,and can be used for the extraction and qualitative and quantitative analysis of gallic acid.
出处
《化工时刊》
CAS
2011年第1期36-38,41,共4页
Chemical Industry Times
