CD 44s和CD 44v6在涎腺腺样囊性癌中的表达

The expression of CD44s and CD44v6 in human salivary adenoid cystic carcinoma *

目的 研究细胞粘附分子 Ｃ Ｄ４４ｓ（ Ｃ Ｄ４４ 标准型）及其剪接变异体 Ｃ Ｄ４４ｖ６ 在涎腺腺样囊性癌（ａｄｅｎｏｉｄ ｃｙｓｔｉｃ ｃａｒｃｉｎｏｍ ａ； Ａ Ｃ Ｃ）中的表达，探讨 Ｃ Ｄ４４ｓ 和 Ｃ Ｄ４４ｖ６ 与 Ａ Ｃ Ｃ生物学特性之间的关系。方法 采用免疫组化方法对 ５０ 例涎腺 Ａ Ｃ Ｃ 标本和１０ 例正常涎腺组织进行 Ｃ Ｄ４４ｓ 和 Ｃ Ｄ４４ｖ６ 的检测。结果 （１） Ｃ Ｄ４４ｓ 和 Ｃ Ｄ４４ｖ６ 在正常涎腺组织均为阳性表达；在 ５０ 例 Ａ Ｃ Ｃ 标本中 Ｃ Ｄ４４ｓ 阳性率为 ５８％ （２９ 例）， Ｃ Ｄ４４ｖ６ 阳性率为７０％ （３５ 例）。（２）涎腺 Ａ Ｃ Ｃ筛状型、管状型和实体型相比较， Ｃ Ｄ４４ｓ 和 Ｃ Ｄ４４ｖ６ 在实体型中表达最差，与其余两组相比较有显著差异（ Ｐ＜ ００５）。（３）在 ２２ 例术后局部有复发的涎腺腺样囊性癌（ａｄｅｎｏｉｄ ｃｙｓｔｉｃ ｃａｒｃｉｎｏｍ ａ； Ａ Ｃ Ｃ），其原发灶 Ｃ Ｄ４４ｓ 阳性率仅为１３６％ （３／２２）。结论 （１） Ｃ Ｄ４４ｓ 及 Ｃ Ｄ４４ｖ６的存在可能与涎腺正常组织结构和功能的维持有关，它们的表达下降或缺失可能和涎腺 Ａ Ｃ Ｃ肿瘤亚型的分化有关；（２） Ｃ Ｄ４４ｓ 的表达下降?

Objective The present study was to investigate the expression of CD44s and CD44v6 in human salivary adenoid cystic carcinoma (ACC) and to discuss the relationship between the expression of the proteins and the biological characteristic of ACC.Methods Formalin fixed,paraffin embedded sections in 10 cases of normal salivary glands and 50 cases of human salivary ACC were examined using immunostaining for CD44s and CD44v6.Results (1)Positive signals of CD44s and CD44v6 were detected in all the specimens of normal salivary glands.Positive signals of CD44s and CD44v6 were detected in 29(58%) and 35(70%) of the 50 cases of ACC.(2)The expression of CD44s and CD44v6 in the solid pattern of ACC was significantly less than that in the cribriform and tubular pattern of ACC( P <0.05).(3)Positive CD44s staining was detected in 3(13.6%) of the 22 ACC primary sites which had a local recurrence after operation.Conclusion (1)Adhesion molecules including CD44s and CD44v6 may contribute to maintain the normal structures in salivary gland tissues.(2)The down regulation or loss of CD44s expression was contributed to the recurrence of human salivary ACC and CD44s could be regarded as one of the factors to evaluate the prognosis of ACC patients.