细胞粘附蛋白在涎腺腺样囊性癌细胞中的表达

The expression of adhesion molecules in human salivary adenoid cystic carcinoma cell line

目的 探讨具有不同转移能力的涎腺腺样囊性癌（ａｄｅｎｏｉｄ ｃｙｓｔｉｃ ｃａｒｃｉｎｏｍ ａ； Ａ Ｃ Ｃ）细胞系中粘附分子表达的差异。方法 采用免疫组化方法对两株人腺样囊性癌细胞系（ Ａ Ｃ Ｃ２ 和 Ａ Ｃ Ｃ Ｍ）培养细胞及其 Ｓ Ｃ Ｉ Ｄ 小鼠移植瘤模型进行粘附分子 Ｃ Ｄ４４ｓ（标准型）、 Ｃ Ｄ４４ｖ６（变异型）、 Ｅｃａｄｈｅｒｉｎ（ Ｅｃａｄ）、αｃａｔｅｎｉｎ（αｃａｔ）、βｃａｔｅｎｉｎ（βｃａｔ）的检测。结果 （１） Ａ Ｃ Ｃ肺转移灶的肿瘤细胞中粘附分子的表达普遍高于其皮下移植瘤细胞；（２） Ａ Ｃ Ｃ Ｍ 肺转移灶肿瘤生长缓慢，细胞分化相对较好，而其皮下移植瘤和腹腔种植瘤肿块生长迅速，细胞分化差；（３） Ｃ Ｄ４４ｖ６ 在 Ａ Ｃ Ｃ Ｍ 和 Ａ Ｃ Ｃ２ 体外培养细胞中均有弱阳性表达，而在异体移植瘤中包括皮下移植瘤和肺部转移灶均无表达。结论 Ａ Ｃ Ｃ细胞中 Ｃ Ｄ４４ｓ 的表达受细胞分化程度影响，并影响了肿瘤细胞本身的生长状况； Ｅｃａｄ／ｃａｔ复合体的表达与否或表达强度与肿瘤细胞的分化成正比； Ｃ Ｄ４４ｖ６ 在涎腺 Ａ Ｃ Ｃ中的表达和转移能力可能无关。

Objective The aim of this study was to investigate the expression of the adhesion molecules in human salivary adenoid cystic carcinoma(ACC) cell lines (ACC 2 line and ACC M strain) which had different metastatic ability.Methods The expression of adhesion molecules,which included CD44s,CD44v6,E cadherin,alph catenin and beta catenin in two human salivary ACC cell lines and Scid mice neoplasm models was investigated by immunohistochemistry.Results (1)Positive staining of adhesion molecules in lung metastatic foci of ACC was higher than that in subcutaneous implanted tumor mass.(2)The growing of ACC M was slower in the cells of abdominal cavity implanted tumor,and also the differentiation of the former was better than the latter.(3)Weaker Positive staining of CD44v6 in ACC cells in vitro was showed,however,no positive staining was detected in lung metastatic foci cells of ACC M and the cells of subcutaneous tumor.Conclusion The expression of CD44s in ACC cells was influenced by cell differentiation,and may affect the growth conditions;The expression levels of E cad/cat was positively correlative to the differentiation of ACC tumor cells;No significant correlation was revealed between the expression of CD44v6 and metastatic ability of ACC.