蛔虫提取物对小鼠Lewis肺癌细胞的细胞毒性作用

Cytotoxic action of a whole worm extract of Ascaris lumbricoides on LLC

目的探讨蛔虫提取物对小鼠Lewis肺癌细胞(LLC)的细胞毒作用。方法选用8种浓度的蛔虫提取物诱导小鼠Lewis肺癌细胞,分别在诱导后24、48、72 h采用四氮唑盐酶还原法(MTT)测A492值,计算细胞存活率和抑制率。结果不同浓度的蛔虫提取物分别作用LLC细胞24、48和72 h,对LLC细胞的增殖均有明显的抑制作用,呈剂量依赖,且以48 h抑制作用最强,之后随着作用时间的延长,BEAL对肿瘤细胞的抑制作用降低。结论蛔虫提取物对小鼠Lewis肺癌细胞具有细胞毒性作用,能够诱导LLC细胞发生凋亡。

Objective To investigate the cytotoxic action of a whole worm extract of Ascaris lumbricoides（BEAL） on a lewis lung carcinoma cell（LLC） line in vitro.Methods Eight concentrations of whole worm extract of A.lumbricoides were chosen to induce cytotoxicity in LLC.Twenty-four,48,and 72 h after induction,the cell survival rate and inhibition rate were calculated according to the A492 results of a microculture tetrazolium test（MTT）.Results Twenty-four,48,and 72 h after induction with different concentrations of whole worm extract of A.lumbricoides,the inhibition rate behaved in a concentration-dependent manner.The inhibition rate peaked 48 h after induction but decreased afterwards.Conclusion A whole worm extract of A.lumbricoides had varying levels of cytotoxicity on LLC and induced cell apoptosis.