高效液相色谱-串联质谱法同时测定食品中五种黄色化工染料

Simultaneous determination of five yellow dyes in foods by high performance liquid chromatography coupled with tandem mass spectrometry

采用高效液相色谱-串联质谱法（HPLC-MS/MS）建立了食品中非法添加的碱性橙、碱性嫩黄、酸性橙Ⅰ、酸性橙Ⅱ和酸性黄36这5种黄色工业染料的定量定性分析方法。使用Agilent ODS C18分离柱（50mm×2.0mm,1.8μm）,以5mmol/L乙酸铵水溶液（0.1%甲酸）-乙腈（3∶2,v/v）为流动相,流速为0.3mL/min。采用电喷雾离子化源,以多反应监测（MRM）方式分别在正、负离子模式下进行检测。在最佳检测条件下,得到了较宽的线性范围和较低的定量检出限。碱性橙和碱性嫩黄的线性范围均为5.0-80.0mg/L;酸性橙Ⅰ、酸性橙Ⅱ及酸性黄36的线性范围均为10.0-160.0μg/L。食品中碱性橙、碱性嫩黄、酸性橙Ⅰ、酸性橙Ⅱ及酸性黄36的定量限分别为20、20、40、40、40ng/g。该方法重现性较好,保留时间和峰面积的相对标准偏差分别不大于0.50%和2.14%。本研究还测定了鸡肉、豆制品和黄鱼中添加的5种化工染料,回收率在79.8%-95.2%之间,结果令人满意。

A method based on high performance liquid chromatography coupled with tandem mass spectrometry（HPLC-MS/MS） was developed for simultaneous determination of five yellow industrial dyes.The separations were performed with an Agilent ODS C18 column at a flow rate of 0.3 mL/min.The mobile phase was 5 mmol/L ammonium acetate（containing 0.1% formic acid）-acetonitrile（3∶2,v/v）.Under the optimized detection conditions,the linear ranges for Chrysoidine G and Basic Yellow 2 were 5.0-80.0 mg/L,and for Acid Orange Ⅰ,Acid orangeⅡ and Acid Yellow 36 were 10.0-160.0 μg/L.The limits of quantification for Chrysoidine G,Basic Yellow 2,Acid Orange Ⅰ,Acid OrangeⅡ and Acid Yellow 36 were 20,20,40,40 and 40 ng/g,respectively.The relative standard deviations of reproducibility of this method for retention time and peak area were no more than 0.50% and 2.14%,respectively.The method was applied to determine the recoveries of the above five dyes in chicken,bean products and yellow croaker were between 79.8%-95.2% with satisfactory results.