结直肠癌组织中基质金属蛋白酶-10的表达及临床意义

Expression of Matrix Matalloproteinase-10 in Colorectal Carcinoma and Its Clinical Significance

目的研究基质金属蛋白酶-10（MMP-10）在结直肠癌组织中的表达情况,分析其与临床病理特征的关系及其在转移中的意义。方法应用免疫组化法检测68例结直肠癌和12例正常黏膜组织MMP-10的表达,并分析其表达与临床病理参数的关系。结果 68例结直肠癌组织中的MMP-10阳性表达率为69.1%,对照组均无阳性表达。伴有淋巴结转移者MMP-10阳性表达率为82.9%,无淋巴结转移者为54.5%,差异有统计学意义（P〈0.05）;腹腔转移阳性者阳性表达率为91.7%,阴性者为64.3%,差异有统计学意义（P〈0.05）;TNM分期Ⅰ-Ⅱ期者阳性表达率为60.6%,Ⅲ-Ⅳ期者为82.9%,差异有统计学意义（P〈0.05）;侵透浆膜者阳性表达率为77.8%,未侵透浆膜者为52.2%,差异有统计学意义（P〈0.05）。免疫组化染色示正常黏膜MMP-10呈阴性表达;原发肿瘤组织呈阳性表达;淋巴结转移灶中MMP-10的表达强度与原发灶中一致。肝转移患者的转移灶周围肝组织均强烈表达,而转移灶本身表达较原发灶弱。结论 MMP-10在结直肠癌组织中高表达。MMP-10阳性表达与肿瘤的发生、侵袭和转移密切相关,在腹腔转移中起重要作用,可作为判断结直肠癌恶性行为的重要生物学标记。

Objective To explore the expression of matrix matalloproteinase-10（MMP-10） in colorectal carcinoma and its clinical significance.Methods The expressions of MMP-10 in 68 colorectal carcinoma tissues and 12 normal colonic mucosa specimens were detected using immunohistochemical methods,and its relationship with clinical pathological parameters were analyzed.Results The positive rate of MMP-10 in 68 primary colorectal carcinoma tissues was 69.1%,while no expression of MMP-10 was detected in the normal colonic mucosa.The positive rate of MMP-10 was 82.9% in the colorectal carcinoma tissues with lymph node metastasis and was 54.5% in those without lymph node metastasis（P〈0.05）.The positive rate of MMP-10 was 91.7% in the colorectal carcinoma tissues with peritoneal implantation and was 64.3% in those without peritoneal implantation（P〈0.05）.The positive rate of MMP-10 was 60.6% in StageⅠ-Ⅱ colorectal carcinoma tissues and was 82.9% in Stage Ⅲ-Ⅳ colorectal carcinoma tissues（P〈0.05）.The positive rate of MMP-10 was 77.8% in the colorectal carcinoma tissues with serous membrane invasion and was 52.2% in those without serous membrane invasion（P〈0.05）.Immunohistochemical staining showed that the expression of MMP-10 was negative in normal mucosa and positive in primary tumor tissues.The expression intensity of MMP-10 in the metastastic lymph nodes was consistent with that in the primary tumor.Surrounding hepatic metastasis also strongly expressed MMP-10,while the expression of MMP-10 in the metastatic lesions was inferior to that in the primary tumor tissues.Conclusion MMP-10 is highly expressed in colorectal carcinoma tissues.The positve expression of MMP-10 is closely correlated with the occurrence,progression,invasion,and metastasis of colorectal cancer,and therefore plays a key role in abdominal cavity metastasis.MMP-10 can be a metastatic predictor and prognostic marker for colorectal cancer.