幽门螺杆菌培养滤液长期作用下大鼠胃粘膜组织学的变化

Longterm effects of concentrated Helicobacter pylori culture supernatant on gastric mucosa of rats

目的 观察幽门螺杆菌（ Ｈｐ） 培养滤液长期作用下大鼠胃粘膜组织学变化．方法 以２－５ ｇ／ Ｌ和５ ｇ／ Ｌ两种浓度的 Ｈｐ 培养滤液让大鼠自由饮用，观察不同时间大鼠胃粘膜厚度、壁细胞数、胃粘膜ＰＡＳ染色阳性层厚度，表面粘液含量的变化及肠上皮化生和异型增生的发生率．结果 实验５ ｗ ｋ 时，实验组大鼠即出现明显的胃粘膜厚度变薄，壁细胞减少，ＰＡＳ 染色阳性层变薄，表面粘液含量减少和ＤＮＡ 合成速率加快，且随着实验时间的延长，上述改变进一步加重． 至５２ ｗｋ 实验结束时，５ ｇ／ Ｌ Ｈｐ 培养滤液组大鼠腺胃粘膜肠化生，硫酸粘液阳性肠化生和异型增生的发生率（５７－１ ％ ，３９－３ ％ ，１７－８ ％ ） 显著高于２－５ ｇ／ Ｌ Ｈｐ 培养滤液组（２７－６ ％ ，１０－３ ％ ，３－４ ％ ） 和正常对照组（６－９ ％ ，０－０ ％ ，０－０ ％ ） ．结论 Ｈｐ 的毒性产物在萎缩性胃炎，肠上皮化生，异型增生乃至胃癌的发生中可能起重要作用．

AIM To investigate the long term effect of concentrated Helicobacter pylori culture supernatant (CHCS) on gastric mucosa of rats. METHODS After 2 5*!g/*!L , 5*!g/*!L CHCS in drinking water were administered orally for 52 weeks, the changes of mucosal thickness, parietal cell number, PAS positive staining layer, mucosal adherent mucus amount, DNA synthesis rate and precancerous lesions incidence in different periods were examined. RESULTS Decreased mucosal thickness, parietal cell number, PAS positive staining layer and amount of mucosal adherent mucus, and increased DNA synthesis were found after five weeks of CHCS feeding in a dose and time dependent manner. By the end of the experiment at the 52nd week, the incidence of intestinal metaplasia (IM), sulfomucin positive IM and dysplasia in 5*!g/*!L CHCS group (57 1%,39 3%,17 8% respectively) were significantly higher than those in 2 5*!g/*!L CHCS group (27 6%,10 3%,3 4% respectively) and control group (6 9%,0 0%,0 0% respectively). CONCLUSION Hp cytotoxins may play an etiological role in the formation of chronic atrophic gastritis, intestinal metaplasia, dysplasia and even gastric carcinoma.