摘要
目的 从分泌抗体的胰腺癌杂交瘤YPC3细胞扩增单抗重链可变区基因。方法 以一步法提取杂交瘤细胞RNA,通过逆转录及聚合酶链式反应,反应产物以琼脂糖凝胶电泳观察。结果 扩增的基因长度为350bp,对照骨髓瘤细胞SP2/0未见扩增。
Objective To amplify the heavy chain variable gene from a hybridoma YPC3 cell line.Methods Total RNA was isolated by a single rapid step of guanidinium extraction. Reverse transcription and polymerase chain reaction were used to amplify the heavy chain variable gene. Biochemical characterization of the gene was showed by gel electrophoresis.Results A strong amplification of a 350 bp DNA segment was found in hybridoma YPC3 while not any amplification in SP2/0 mouse myeloma cells.Conclusion Successful amplification of the heavy chain variable gene makes it possible to generate engineering antibody to pancreatic carcinoma.
出处
《广东医学》
CAS
CSCD
1999年第10期749-750,共2页
Guangdong Medical Journal
基金
卫生部临床学科重点项目资助
关键词
胰腺肿瘤
单克隆抗体
基因扩增
PCR
Pancreatic neoplasm\ Antibody, monoclonal\ Gene\ Polymerase chain reaction