针药并用对帕金森病小鼠纹状体DA、NE和5-羟色胺的影响

Effect of Combined Acupuncture and Medicine on Striatal DA,NE and 5-hydroxytryptamine in a Mouse Model of Parkinson Disease

目的观察平颤解郁方配合针刺治疗对帕金森病模型小鼠纹状体DA、NE和5-HT等神经递质的影响。方法将成年C_(57)/BL雄性小鼠28只随机分为正常组、模型组、西药组和针药组4组,每组7只。采用高效液相色谱-电化学检测法,测定各组小鼠纹状体DA、NE和5-羟色胺(5-HT)的含量。结果模型组、西药组和针药组小鼠纹状体DA、NE、5-HT含量均显著低于正常组(P<0.01,P<0.05),西药组和针药组小鼠纹状体DA、NE、5-HT含量显著高于模型组(P<0.01,P<0.05)。结论平颤解郁方配合针刺治疗可提高纹状体下降的DA、NE、5-HT水平。

Objective To investigate the effect of tremor and depression-relieving prescription plus acupuncture on striatal neurotransmitters DA, NE and 5-HT in a mouse model of Parkinson disease. Methods Twenty-eight adult male C57/BL mice were randomly allocated to four groups： normal, model, Western drug and combined acupuncture and medicine, 7 mice each. Striatal DA, NE and 5-hydroxytryptamine （5-HT） contents were measured by high efficiency liquid chromatography-electrochemical determination in every group of mice. Results Striatal DA, NE and 5-hydroxytryptamine （5-HT） contents were significantly lower in the model, Western drug and combined acupuncture and medicine groups of mice than in the normal group （P〈0.01,P〈0.05） and significantly higher in the Western drug and combined acupuncture and medicine groups of mice than in the model group （P〈0.01, P〈0.05）. Conclusion Tremor and depression-relieving prescription combined with acupuncture can raise low striatal DA, NE and 5-HT levels.