超高效液相色谱法同时测定食品接触材料与食品模拟物中6种紫外吸收剂

Simultaneous Determination of 6 Ultraviolet Absorbers in Food Contact Materials and Food Simulants by Ultra Performance Liquid Chromatography

建立了食品接触材料（聚氯乙烯和聚乙烯塑料）和食品模拟物中6种紫外吸收剂（UV-9、VV-326、UV-327、uv-329、uv-234和uv-1577）的超高效液相色谱测定方法。采用四氢呋喃溶解聚氯乙烯、热甲苯溶解聚乙烯材料以提取样品中的目标化合物；用凝胶渗透色谱法净化食品模拟物橄榄油样品；在ACQUITYUP—LCBEHC18（1．7μm×2．1mm×100mm）反相色谱柱上以乙腈-0．1％乙酸水溶液为流动相，流速为0．3mL/min的梯度程序使6种紫外吸收剂得到了有效分离；于300nm波长下外标法定量。6种紫外吸收剂在0．01～10mg／L的质量浓度范围内呈良好线性，相关系数r≥0．9995。方法的定量下限（S／N=10）为0．01mg／L，加标回收率为93％-99％，相对标准偏差为1．3％～4．0％。该方法操作简单、灵敏度高、重复性好，适用于食品接触材料及食品模拟物中6种紫外吸收剂的同时测定，已用于实际样品的检测。

An ultra performance liquid chromatographic （UPLC） method was developed for the simultaneous determination of 6 ultraviolet absorbers including UV-9, UV-326, UV-327, UV-329, UV- 234 and UV-1577, in food contact materials（polyvinyl chloride and polyethylene plastics） and food simulants. The target compounds were extracted with polyvinyl chloride materials dissolved in tetrahydrofuran or polyethylene materials dissolved in hot toluene. The food simulants olive oil samples were cleaned up by gel permeation chromatography （GPC）. The separation of 6 ultraviolet absorbers was performed on an ACQUITY UPLC BEH Cis （ 1.7 μm × 2. 1 mm× 100 mm） column by gradient elution using 0. 1% acetic acid - acetonitrile as mobile phase. The flow rate was set at 0. 3 mL/min. The quantitative determination of tatget compound was carried out by the external standard at a detection wavelength of 300 nm. The calibration curves of 6 analytes were linear in the range of 0.01 - 10 mg/L with correlation coefficients not less than 0. 999 5. The limits of quantitation（S/N = 10） were 0. 01 mg/L. The spiked recoveries at different spiked levels were in the range of 93% - 99% with relative standard deviations of 1.3% -4. 0% . The method was simple, sensitive and reproducible, and was suitable for the simultaneous determination of 6 ultraviolet absorbers in food contact materials and food simulants.