禽病原性大肠杆菌6种血清型分离株外膜蛋白型的研究

Studies on Outer Membrane Protein Patterns of 6 Kinds of Serotypes of Pathogenic Escherichia Coli from Poultry

从病鸡中分离的６ 种血清型（ Ｏ２、 Ｏ４６、 Ｏ１８、 Ｏ１２１、 Ｏ７６、 Ｏ１３１）及５ 种标准血清型（ Ｏ１、 Ｏ１５、 Ｏ３５、 Ｏ３６、 Ｏ７８）大肠杆菌中提取外膜蛋白（ Ｏｕｔｅｒ ｍ ｅｍ ｂｒａｎｅ ｐｒｏｔｅｉｎ），经 Ｓ Ｄ Ｓ－ Ｐ Ａ Ｇ Ｅ 分析表明，分离株 Ｏ７６、 Ｏ１３１及标准株 Ｏ１、 Ｏ３５的外膜蛋白型由２ 条带组成，为 Ｏ Ｍ Ｐ－ １ 型；分离株 Ｏ２、 Ｏ４６、 Ｏ１８、 Ｏ１２１和标准株 Ｏ１５、 Ｏ３６、 Ｏ７８的外膜蛋白型由３ 条带组成，为 Ｏ Ｍ Ｐ－ ２ 型，结果表明，不同血清型菌株之间有相同的 Ｏ Ｍ Ｐ型。

Outer membrane proteins were isolated from (O 2 、O 46 、O 18 、O 121 、O 76 、O 131 )six serotypes of Pathosenic Escherichia Coli from Poultry and (O 1 、O 15 、O 35 、O 36 、O 78 )five standard strains.SDS PAGE analysis showed that OMP pattern of O 76 、O 131 isolates and O 1、O 35 standard strains consisted of two protein bands and were named OMP 1 pattern,OMP pattern of O 2、O 46 、O 18 、O 121 isolates and O 15 、O 36 、O 78 standard strains had three protein bands and were named OMP 2 pattern. The result shows that strains of different serotypes have the same OMP pattern