白花蛇舌草中黄酮提取工艺的优化及其结构鉴定

Optimization of Extraction Process of Flavonoids from Hedyotis diffusa and Identification of Its Structure

[目的]采用微波辅助提取白花蛇舌草(Hedyotis diffusa)中的黄酮类化合物,并优化其提取工艺,对提取物的化学结构进行鉴定,为白花蛇舌草药用价值的进一步开发提供参考。[方法]用正交试验优化白花蛇舌草黄酮类化合物的最佳提取工艺条件,通过紫外光谱、红外光谱等对提取物的化学结构进行表征。[结果]影响黄酮提取率的因素主次顺序为:提取温度>乙醇浓度>提取时间>料液比;最佳提取工艺为:乙醇浓度为80%,提取时间为40min,料液比为1∶40,提取温度为80℃,在此条件下白花蛇舌草中黄酮的提取率达2.86%。白花蛇舌草的黄酮类化合物主要成分为黄酮醇类。[结论]该研究为白花蛇舌草药用价值的进一步开发提供了参考。

[Objective]To optimize the extraction process of flavonoids from Hedyotis diffusa and identify its structure,so as to provide reference for the further development of the medicinal value of Hedyotis diffusa. [Method] By employing single factor test and orthogonal test,the extraction process of flavonoids from Hedyotis diffusa was opitimized. Its chemical structure was identified by UV and IR analysis. [Result] The influencing order of each factor on the yield of flavonoids was determined to be extraction temperatureconcentration of ethanol extraction durationsolid to liquid ratio,and the optimal extraction conditions were as follows：80% of ethanol concentration,40 min of extraction duration,1∶40 of solid to liquid ratio and 80 ℃ of extraction temperature. The yield of flavonoids from Hedyotis diffusa was up to 2.86% under the optimum extraction conditions. The flavonoids from Hedyotis diffusa were rich in flavonol. [Conclusion] The study provides reference for the further development of the medicinal value of Hedyotis diffusa.