一种简单实用的单细胞分离技术

A simple and effective method for single cells separation

背景:基于单细胞水平的研究是干细胞、肿瘤干细胞研究的发展趋势。许多研究者常常因为无法轻易获得足够数量的单细胞而在研究时望而却步。掌握简单有效的单细胞分离技术为准确、定量的单细胞水平研究奠定基础。目的:介绍一种简单有效的单细胞分离技术。方法:在显微操作法的基础上改进,利用改制小号的静脉注射静脉注射针及微量移液器组合制成单细胞分离种植装置并采取双手操作法进行单细胞分离。采用染色细胞分离的方法对该技术分离单细胞的效率进行验证。结果与结论:单细胞分离的成功率达到96.5%。利用该技术分离单细胞具有取材容易、制作简单、操作直观可靠、分离成功率高、易于推广普及的特点。分离的单细胞可直接用于进一步的研究。

BACKGROUND：Research of stem cell or cancer stem cell at single cell level would be popularity in the near further.Many researchers stop going ahead when they have no idea to obtain lots of single cells in their study.A simple and effective method for single cell separation would be a foundation of exactly and quantitative study at single cell level.OBJECTIVE：To introduce a simple and effective method for single cell separation.METHODS：The separation method was improved based on micromanipulate and single cells were separated by using special separation equipment which makes up of micropipettor and the smallest intravenous needle.Success rates of separation were evaluated by separated staining of single cells.RESULTS AND CONCLUSION：Success rate of single cells separation was 96.5%.The method has many advantages：material was easily to find;the manipulate method was simple,visual,reliable and easy to popularization and application;high success rate.Those single cells which had been separated can be used for further study directly.