骨髓间充质干细胞向软骨的诱导分化

Induced differentiation of bone marrow mesenchymal stem cells into chondrocytes

背景:骨髓间充质干细胞在体外不同环境及生物因子的作用下,可以转化为软骨细胞,是目前软骨组织工程领域研究的重点。目的:对近年来细胞因子、应力刺激、三维空间结构在骨髓间充质干细胞向软骨细胞分化过程中的作用作一综述。方法:应用计算机检索CNKI和Pubmed数据库中2001-01/2010-06关于骨髓间充质干细胞分化为软骨细胞的文章,在标题和摘要中以"骨髓间充质干细胞,软骨细胞,诱导分化"或"bone marrow mesenchymal stem cells,chondrocyte,differentiation"为检索词进行检索。选择文章内容与骨髓间充质干细胞分化为软骨细胞有关者,同一领域文献则选择近期发表或发表在权威杂志文章。初检得到109篇文献,根据纳入标准选择关于骨髓间充质干细胞分化为软骨细胞的26篇文章进行综述。结果与结论:细胞因子、应力刺激、三维空间结构等因素影响骨髓间充质干细胞向软骨细胞分化,优化这些因素的条件,可以更容易分化出软骨细胞表型。随着骨髓间充质干细胞分化为软骨细胞的深入研究,这些因素的应用必将更为广泛。

BACKGROUND：Bone marrow mesenchymal stem cells（BMSCs） affected by different environmental and biological factors in vitro can transform into cartilage cells,which is the focus of cartilage tissue engineering study in recent years.OBJECTIVE：To summarize the effects of cytokines,stress stimulation,three-dimensional structure on BMSCs differentiation into cartilage cells.METHODS：The databases of China National Knowledge Infrastructure and Pubmed were searched for the articles about BMSCs differentiation into chondrocytes published from January 2001 to June 2010.The Key words were ＂bone marrow mesenchymal stem cells,chondrocyte,differentiation＂,which used by inputting in titles and abstracts.The articles addressing BMSCs differentiation into chondrocytes were included.Those in the same field published recently or in authorized journals were included.There were 109 articles after the initial survey.Totally,26 articles were included in accordance with inclusion criteria. RESULTS AND CONCLUSION：Cytokines,stress stimulation,three-dimensional structure effects on BMSCs differentiation into cartilage cells was significant.The conditions for optimizing these factors can be more easily induced to differentiate into chondrocyte phenotype.With the deep understanding of BMSCs differentiation into cartilage cells,the application of these factors will be more extensive.