异常应力条件下关节软骨的变化

Variation of articular cartilages under abnormal stress

背景:关节软骨细胞的正常生理功能与其受到的正常应力刺激密切相关,研究应力在关节软骨退行性变中的作用对临床治疗软骨退行性改变具有重要意义。目的:观察兔膝关节软骨在异常应力条件下的变化,探讨应力对于关节软骨正常生长代谢以及修复过程的影响。方法:将42只新西兰大白兔随机分为对照组和制动2,4,6周组。采用兔伸膝制动动物模型,对制动2,4,6周及正常兔的膝关节全层软骨进行苏木精-伊红染色及Ⅱ型胶原免疫组织化学染色,比较制动前后软骨组织学、病理积分及Ⅱ型胶原分泌的变化情况。结果与结论:随着制动时间的延长,关节软骨的退变程度逐渐加深,病理积分结果显示各组间差异具有显著性意义(P<0.01)。免疫组织化学染色结果显示随着制动时间的延长,软骨细胞分泌的Ⅱ型胶原先增多,后减少。说明异常应力可影响兔关节软骨细胞正常生理活动及其分泌Ⅱ型胶原的能力。

BACKGROUND：The normal function of articular cartilages is closely associated with normal stress stimulation.The study of effect of stress on articular cartilage degeneration plays an important role for the treatment of articular cartilage degeneration in clinic.OBJECTIVE：To study the characteristic of articular cartilage degeneration under abnormal stress,and to explore the effect of stress on normal growth and repair of articular cartilage.METHODS：Totally 42 New Zealand white rabbits were randomly divided into the control and 2-,4-and 6-week immobilization groups,all of them were immobilized the right hind limb with plaster bandage in extension position except those in the control group.The articular cartilage of the lateral tibial plateau was harvested for hematoxylin-eosin staining and immunohistochemistry to observe the pathological change and the content of type Ⅱ collagen.RESULTS AND CONCLUSION：The articular cartilage appeared destruction and erosion aggravated with time prolonged,and the pathological scores showed there were significant differences among groups（P 0.01）.Immunohistochemistry results showed that the articular cartilage secreted type Ⅱ collagen was increased followed by decreased with immobilization time prolonged.Abnormal stress can lead to articular cartilage degeneration and affect type Ⅱ collagen secretion.