葛根素联合雌二醇干预去卵巢大鼠骨组织和骨代谢的变化

Effect of puerarin combined with estradiol on bone tissues and bone metabolism in ovariectomized rats

背景:用中药与雌激素治疗骨质疏松症,可能减少激素用量,进而减少不良反应和并发症的发生。目的:观察小剂量葛根素联合雌二醇对去卵巢大鼠的骨组织和骨代谢的影响。方法:5月龄健康雌性大白鼠分为假手术组、去卵巢模型组、葛根素组、雌二醇组和小剂量葛根素+雌二醇组,建立去卵巢动物模型,分别皮下注射雌二醇100μg/kg,2次/周和/或葛根素25mg/kg,1次/d。在第4,8,12和20周,每组随机取6只大鼠取股骨切片观察骨组织,采血测量血钙、磷和碱性磷酸酶。结果与结论:去卵巢模型组第4,8周与假手术组相比血钙、磷明显降低(P<0.01),血碱性磷酸酶均明显升高(P<0.01),骨组织呈骨质疏松的病理改变。3个用药组与假手术组相比骨组织和血钙、磷和碱性磷酸酶无明显差异(P<0.05)。小剂量葛根素+雌二醇组与葛根素组或雌二醇组相比骨组织和血钙、磷和碱性磷酸酶无明显差异(P>0.05)。小剂量的葛根素联合雌二醇对去卵巢大鼠的骨质疏松症的治疗效果与单独使用较大剂量的葛根素或较大剂量的雌二醇效果相近。

BACKGROUND：Chinese medication combined with estradiol can be used to treat osteoporosis,which may reduce estradiol dose,decrease the adverse reaction and complication.OBJECTIVE：To observe the effects of small dose of puerarin combined with estradiol on bone tissues and bone metabolism in ovariectomized rats.METHODS：5-month-old healthy female rats were divided into five experimental groups：sham-operated,model,puerarin,estradiol and low-dose puerarin ＋ estradiol groups.All rats were prepared for ovariectomized models except those in the sham-operated group.Totally 100 μg/kg estradiol were subcutaneous injected into rats,twice per week,or 25 mg/kg puerarin were injected,once per day.The bone tissues were observed and the serum calcium,phosphorus and alkaline phosphatase（ALP）levels were measured at 4,8,12 and 20 weeks after operation.RESULTS AND CONCLUSION：Compared with the sham-operated group,the serum calcium and phosphorus of the model group was decreased significant at 4 and 8 weeks after operation（P 0.01）,but the ALP levels were notably increased（P 0.01）,the bone tissues presented with osteoporosis.Compared with the sham-operated group,there were no significances among the three medication groups in the serum calcium,phosphorus and ALP levels（P 0.05）.In addition,there was no significance intergroup comparison among three treatment groups（P 0.05）.The small dose of puerarin ＋ estradiol shows similar effects to large single doses of puerarin or estradiol on the treatment of rat ovariectomized osteoporosis.