小鼠肝脏端粒酶活性与α-亚麻酸、维生素复合剂的干预

Effects of alpha-linolenic acid and vitamin compound on telomerase activation in mouse liver

背景:α-亚麻酸和维生素A、C、E均具有抗衰老作用,但具体机制不明。研究表明增加端粒酶的活性可预防细胞过早老化。联合应用α-亚麻酸与维生素是否可通过增强端粒酶活性起到抗衰老作用的研究鲜有报道。目的:通过联合应用α-亚麻酸与维生素A、C、E复合剂,观察肝脏端粒酶mRNA表达变化、端粒酶活性和端粒缩短速度,探讨联合应用α-亚麻酸与维生素是否可通过增强端粒酶活性起到延缓衰老的作用。方法:22月龄清洁级老年昆明小鼠,随机分成6组:老年小鼠对照组,α-亚麻酸组,α-亚麻酸+维生素E组,α-亚麻酸+维生素C组,α-亚麻酸+维生素A组,α-亚麻酸+维生素A、C、E组。2月龄清洁级青年昆明小鼠,作为青年小鼠对照组。各组小鼠通过灌胃给药30d后分离肝脏,应用RT-PCR测定端粒酶的mRNA表达。结果与结论:青年与老年小鼠的肝脏均表达端粒酶mRNA,青年小鼠对照组端粒酶mRNA表达水平高于老年小鼠对照组(P<0.05)。5个实验组端粒酶mRNA表达均较老年小鼠对照组明显增高(P<0.01);与青年小鼠对照组比较,α-亚麻酸+维生素E、α-亚麻酸+维生素C,α-亚麻酸+维生素A和α-亚麻酸+维生素A、C、E4组端粒酶mRNA表达均增高(P<0.01)。5个实验组之间相比较,α-亚麻酸+维生素A、C、E组端粒酶mRNA表达明显高于其他4组(P<0.01)。结果表明,α-亚麻酸与维生素A、C、E复合剂可协同提高端粒酶活性,延长端粒长度,进而起到延缓衰老的作用。

BACKGROUND：α-linolenic acid and vitamin A,C,E can prevent aging.However,the mechanism is still not clear.Numerous studies have documented that telomerase can protect cells from aging.In our knowledge,no research showed that compound of linolenic acid and vitamin can protect cells from aging by promoting activity of telomerase.OBJECTIVE：α-linolenic acid and vitamin A,C,E compound was used to observe the telomerase mRNA expression,telomerase activity,and telomere shortening velocity,to explore whether α-linolenic acid and vitamin A,C,E compound can delay aging by promoting activity of telomerase.METHODS：Old mice（22 months old） were grouped randomly：old control group,α-linolenic acid,group;α-linolenic acid ＋ vitamin E group;α-linolenic acid ＋ vitamin C group;α-linolenic acid ＋ vitamin A group;and α-linolenic acid ＋ vitamin E＋A＋C group.2 months old mice were served as young control.All mice livers were taken out at 30 days after intragastric administration for measurement of telomerase mRNA expression by RT-PCR.RESULTS AND CONCLUSION：A higher expression of telomerase mRNA was showed in young mice compared with old control mice（P 0.05）.The expression of telomerase mRNA in all 5 groups treated by α-linolenic acid and/or vitamin was obviously increased compared with old control group（P 0.01）.Compared with the young control group,the expression of telomerase mRNA in all 4 groups treated by α-linolenic acid ＋ vitamin A,vitamin E,vitamin C,and vitamin ACE was obviously increased（P 0.01）.The inter experimental groups comparison showed that,the group treated by α-linolenic acid＋vitamin ACE had a higher expression of telomerase mRNA（P 0.01）.The present study show that,combined application of α-linolenic acid ＋ vitamin A,C,E can enhance activity of telomerase,prevent shorten of telomere and protect cells from aging.