大庆聚驱后油藏微生物群落结构分析

Research of Microbial Community Structure after Polymer Flooding in Daqing Reservoir

目的:进一步研究大庆聚驱后油藏微生物种类。方法:应用T-RFLP方法比较系统地分析比较了聚驱后油藏微生物多样性,分析了与采油功能相关的主要微生物组成,初步研究了聚驱后油藏微生物分布特点及规律。结果:利用聚驱后油藏细菌群落16S rDNA构建克隆文库,共挑取了49个阳性克隆,T-RFLP分型结果,共出现21个不同类型,所得的序列在Genbank中比对的结果表明细菌克隆可分为5个类群,分属于变形杆菌、拟杆菌、脱铁杆菌、厚壁菌以及未确定分类的类群;同时构建了聚驱后油藏古菌群落16S rDNA的克隆文库,共挑取了48个阳性克隆,根据T-RF分型结果共有5个类群,构建的古菌克隆文库的12个阳性克隆分属5个不同的类群,这些克隆和产甲烷菌亲缘关系很近。结论:该研究为大庆油田本源微生物采油技术的应用提供了可靠的理论依据。

Objective：To further study the microbial species of reservoir after polymer flooding in Daqing.Method：Microorganism diversity was systematically analyzed and compared after polymer flooding by T-RFLP technology.Component of oil recovery microbial community had been analyzed.Result：Clone library by 16S rDNA of bacterial community after polymer flooding reservoir was constructed.A total of 49 positive clones were chosen,The T-RFLP result showed that there were 21 different types.The results in Genbank indicated that clones belonged to five different published phylum,Proteobacteria,Bacteriodetes,Deferribacter,Firmicutes and Candidate division OP11;Clone library by 16S rDNA of archaea community after polymer flooding reservoir was constructed,a total of 49 positive clones were chosen,twelve possitive clones in clone library of archaea belonged to five different published phylum,Most of them were similar with Methanotrops.Conclusion： It provided a reliable theoretical basis for the application of indigenous microbial enhanced oil recovery technology.