猪心房Atricure双极射频消融系统消融组织透壁性损伤分析

The application of Atricure bipolar radiofrequency system in ablation of different parts and different times of pig heart atrium and the analysis of transmural lesions

目的 分析Atricure双极射频消融系统对猪心房不同部位不同次数的消融产生的组织透壁性损伤.方法 选取6只新鲜（离体时间＜20 min）的保存完整心房组织的普通家猪心脏,按消融部位分为4组,分别对左心房后壁、左心房顶、右心房前壁以及左心房后壁近二尖瓣后瓣环部位使用Atricure双极射频消融系统进行消融.对各部位心房组织,在长约2.0 cm的厚度均一区域,间隔0.5 cm行4次夹闭消融,记录每次消融结束时间,取各次消融后的心房组织,分别用4%甲醛和5%戊二醛保存,送光镜下和透射电镜下观察组织透壁性损伤情况.对消融时间以及损伤情况行统计学分析、处理.结果 同一部位心房组织,随着消融次数的增加,所需消融时间逐渐降低;不同部位的心房组织,相同次数的消融,所需消融时间与组织厚度呈正比.结论 Atricure双极射频消融系统安全、有效,操作方便.左心房后壁、左心房前壁相对较薄的部位,至少2次消融才能确保组织的透壁性损伤;右心房前壁以及左心房后壁近二尖瓣后瓣环部位,3～4次消融才能确保组织的透壁性损伤.

Objective To analyze the transmural lesions of different parts of the pig heart atrium received different times of ablation applied with Atricure bipolar radiofrequency system. Methods Six fresh （ex vivo time ＜ 20 min） pig hearts with atrium preserved intact were used as the experimental objects and experimental groups were divided according to the ablation position. The Atricure bipolar radiofrequency system was applied in the ablation of the parts of the atrium, such as posterior wall of left atrium, anterior wall of left atrium, anterior wall of right atrium and posterior wall of left atrium close to mitral posterior ring.Ablate the position of the atrium lengthed about 2.0 cm with the same thickness with an interval of 0. 5 cm for 4 times respectively, also recording the time of every ablation. For each part and each time of ablation,the ablated atrial tissue was preserved with 4% formaldehyde and 5% glutaraldehyde, and was sent for observation under light microscope and transmission electron microscope. The ablation time and lesion were analyzed statistically. Results In the same position of the atrium, ablation time decreased with the times of the ablation, in different position of the atrium with same time of ablation, time showed a positive proportion with the thickness of the atrium. Conclusions Atricure bipolar radiofrequency system is very safe and efficient, also convenient for manipulation. With regard to the relatively thinner part of the atrium, such as posterior wall and anterior wall of left atrium, at least two times of ablation can ensure transmural lesion of the atrial tissue, but to the position of the atrium such as anterior wall of right atrium and posterior wall of left atrium close to mitral posterior ring, 3 to 4 times of ablation can ensure transmural lesion of the atrial tissue.