miR-196a-2基因多态性与慢性丙型肝炎抗病毒治疗疗效的关系

miR-196a-2 gene polymorphism and the antiviral therapy of chronic hepatitis C

目的 探讨miR-196a-2 SNP位点rs11614913与慢性丙型肝炎患者对长效干扰素联合利巴韦林治疗疗效的相关性.方法 选择接受聚乙二醇干扰素α-2a（Peg-IFN-α-2a）或α-2b（PegIFN-α-2b）联合利巴韦林（RBV）抗病毒治疗的慢性丙型肝炎患者共139例,其中持续病毒学应答（SVR）组82例,无病毒学应答（NVR）或复发组57例,采集静脉血并进行DNA提取,采用聚合酶链式反应-限制性内切酶长度多态性（PCR-RFLP）方法 对miR-196a-2进行SNP分型,组间差异进行分析比较.结果 位于miR-196a-2 rs11614913位点的CT基因型与TT基因型两组间比较差异具有统计学意义[P=0.006,A值=3.5（1.402-8.737）],CC基因型与TT基因型在两组间比较差异有统计学意义[P值=0.011,A值=4.000（1.330-12.031）].T等位基因与C等位基因频率在两组间比较差异有统计学意义[P=0.008,A值=1.926（1.185-3.131）].结论 位于miR-196a-2基因区rs11614913位点的多态性与慢性丙型肝炎患者抗病毒疗效具有相关性,T等位基因或TT基因型与SVR相关,而C等位基因或CC基因型与NVR或复发相关.

Objective To investigate the relationship between the SNP rs11614913 on miR196a-2 gene and the treatment effects of Peg-IFN-α plus Ribavirin on chronic hepatitis C patients. Methods The total 139 patients of chronic hepatitis C infection who received the treatment of Peg-IFN-α-2a or Peg-IFN-α-2b plus Ribavirin were enrolled in this study. The patients were divided into two groups： sustained virological response（SVR） （ n = 82 ） group and non virological response （ NVR ） or recurrence （ n = 57 ）group. Blood samples were collected and chromosomal DNA was extracted. The miR-196a-2 polymorphism was determined with the method of polymerase chain reaction （PCR）-restriction fragment length polymorphism（RFLP）. Results In our study, there was statistically association between miR-196a-2polymorphism and the antiviral therapy efficacy of hepatitis C patients. There was statistically significance in the CT genotype and the TT genotype of m iR-196a-2 between the two groups [P = 0. 009, A = 2. 924（ 1. 285-6. 652）]. There was statistically significance in the CC genotype and the TT genotype between the two groups [P = 0. 036, A = 3.091 （ 1. 052 - 9. 078 ）]. There was statistically significance in the C allele and the T allele between the two groups [P =0.036, A =3.091（1.052 -9.078）]. Conclusion These findings suggested that the rs11614913 SNP in miR - 196a-2 be associated with the antiviral therapy efficacy of hepatitis C patients, and the TT genotype or T alleles be associated with the SVR while the CC genotype or C allele could be related to the NVR or recurrence.