期刊文献+

流式技术测定乙型肝炎患者外周血特异性CD8+T细胞及其临床意义 被引量:3

Determination of circulating HBV specific CD8 + T cells in hepatitis B patients by flow cytometry and its clinical significance
原文传递
导出
摘要 目的 探讨流式细胞技术检测乙型肝炎患者HBV特异性CD8+T细胞的水平并分析其临床意义.方法 采用HLA-A2分子胞外段与HBV核心表位肽core18-27结合的HLA-肽五聚体(Pentamers)对25例急性乙肝患者、35例慢性乙肝患者和10例正常健康人的外周血进行染色,设计流式细胞技术检测其针对该肽段的特异性CD8+T细胞数量,以占总计数CD8+细胞数的百分比表示.结果 12例HLA-A2+的急性乙肝患者急性期可检测到高水平的特异性CD8+T细胞,中位数为2.93%(1.12%~4.63%),16例HLA-A2+的慢性乙肝患者特异性CD8+T细胞水平较低,中位数为0.75%(<0.01%~1.76%),两组之间差异有统计学意义(P<0.01).10例HLA-A2+正常对照组、13例HLA-A2-急性乙肝对照组和19例HLA-A2慢性乙肝对照组的特异性CD8+T细胞均不超过0.02%.结论 HLA-肽五聚体流式细胞技术能在体外直接检测HBV特异性的CD8+T细胞数量,其水平可能影响着HBV感染者体内病毒的清除,且与乙肝的不同临床转归有关. Objective To explore the levels of circulating HBV specific CD8+ T cells in hepatitis B patients and analysis its clinical significance. Methods HBV specific CD8 + T cells in whole blood samples of twenty-five acute hepatitis B patients, thirty-five chronic hepatitis B patients and ten healthy control were stained with pentamers complex of HLA-A2 and HBV core 18-27 peptide and counted by flow cytometry.Results The medians of HBV core 18-27 specific CD8+ T cells quantities among total CD8+ cells were 2.93% ( 1.12% -0. 63% ) in 12 HLA-A2 + acute hepatitis B patients and 0. 75% ( < 0. 01% - 1.76% ) in 16 HLA-A2+ chronic hepatitis B patients respectively. There was showed a marked significant between the two groups (P < 0. 01 ). The medians of HBV core 18-27 specific CD8+ T cells of 10 HLA-A2+ healthy control, 13 HLA-A2- acute hepatitis B patients and 19 HLA - A2- chronic hepatitis B patients were all lower than 0. 02% separately. Conclusion HLA-peptides pentamers staining flow cytometry is a direct ex vivo method to detect the levels of circulating HBV specific CD8 + T cells which may be play a crucial role in complete clearance of HBV and relates to clinical consequence in hepatitis B patients.
作者 姚永良
出处 《中华实验和临床病毒学杂志》 CAS CSCD 北大核心 2010年第6期476-478,共3页 Chinese Journal of Experimental and Clinical Virology
关键词 肽类 CD8阳性T淋巴细胞 流式细胞术 肝炎 乙型 Peptides CD8-Positive T-Lymphocytes Flow cytometry Hepatitis B
  • 相关文献

参考文献4

  • 1中华医学会传染病与,寄生虫病学分会,肝病学分会.病毒性肝炎防治方案[J].中华肝脏病杂志,2000,8(6):324-329. 被引量:14010
  • 2Webster GJM,Reignat S,Bertoletti A,et al.Incubation Phase of acute hepatitis B in man:dynamic of cellular immune mechanisms.Hepatology,2000,32:1117-1124.
  • 3Franzese O,Kennedy PT,Gehring AJ,et al.Modulation of the CD8+ T-Cell Response by CD4+ CD25+ Regulatory T Cells in Patients with Hepatitis B Virus Infection.J Virol,2005,79:3322-3328.
  • 4Stoop JN,van der Molen RG,Baan CC,et al.Regulatory T cells contribute to the impaired immune response in patients with chronic hepatitis B virus infection.Hepatology,2005,41:771-778.

共引文献14009

同被引文献30

引证文献3

二级引证文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部