硫酸镁对大鼠神经干细胞辐射损伤保护作用的初步研究

Study on the protective effect of MgSO4 on the radiation-induced neural stem cell injury

目的 探讨硫酸镁对大鼠神经干细胞辐射损伤的保护作用.方法 取新生大鼠神经干细胞进行培养,用免疫荧光法进行神经干细胞的鉴定后,用含有不同浓度硫酸镁的培养基培养神经干细胞,选择出硫酸镁的合适浓度为1.25 mg/ml.将神经干细胞分为空白对照组、单纯照射组及照射＋硫酸镁组,照射＋硫酸镁组神经干细胞加入1.25 mg/ml的硫酸镁培养24 h后,分别用2和4 Gy 60Co γ射线对单纯照射组及照射＋硫酸镁组进行照射,分别于照射后24、48和72 h用CCK-8对各组进行神经干细胞增殖情况的检测,同时用流式细胞仪对各组神经于细胞凋亡率进行检测.结果 与空白对照组比较,单纯照射组各时间点反映神经干细胞增殖情况的吸光度值明显降低（t=5.33～8.44,P＜0.05）,神经干细胞的凋亡率明显升高（t=30.60～71.22,P＜0.05）.与单纯照射组比较,照射＋硫酸镁组除24 h时间点外余各时间反映神经于细胞增殖的吸光度值有所上升（t=2.45～4.71,P＜0.05）,各时间点神经干细胞的凋亡率明显降低（t=6.73～41.12,P＜0.05）.结论 硫酸镁具有减轻电离辐射后神经干细胞增殖损伤,降低电离辐射所致的细胞凋亡率.

Objective To explore the neuroprotective effect of magnesium sulfate on radiation induced neural stem cell injury. Methods Brain tissue was obtained from new-born sprague-dawley rats within 24 hours, and the cerebral hemisphere was dissociated to culture the neural stem cells. After being identified by immunofluorescence method, the neural stem cells were randomly divided into 3 groups as blank control group, experimental control group and experimental group. The neural stem cells of experimental control group and experimental group were irradiated with 2 or 4 Gy of gamma rays. The proliferation and the cell cycle of neural stem cells were detected at different time-points ranging from 24 h,48 h, 72 h after irradiation with CCK-8 and FCM. Results Compared with the blank control group, the proliferation rate of experimental control group was significantly reduced （ t = 5. 33-8.44, P ＜ 0. 05 ）, and the G1 phase arrest of experimental control group was significantly enhanced （ t = 30. 60-71.22, P ＜ 0. 05 ）.Compared with the experimental control group, the proliferation of experimental group significantly increased excluding that of 24 h （ t = 2.45-4. 71, P ＜ 0.05 ）, the apoptosis rate of experimental group significantly decreasd （t = 6.73-41.12, P ＜ 0.05 ）, which was closer to the blank control group.Conclusion Magnesium sulfate can alleviate the injury of proliferation and decrease the cell apoptosis in the early stage after irradiation.