mTOR/P70S6K信号通路在小儿血管瘤演变中的作用

The role of mTOR/p70S6K signaling pathway in the development of hemangioma of children

目的 探讨mTOR/p70S6K信号通路在小儿血管瘤的发生、发展及消退过程中的作用.方法 收集未经其他治疗、单纯手术切除并经病理诊断为血管瘤的标本31例,结合Mulliken分类法与增殖细胞核抗原（PCNA）表达对血管瘤进行分类和分期,免疫组织化学检测并比较增生期血管瘤和消退期血管瘤组织中雷帕霉素靶蛋白（mTOR）和p70S6K-a的表达水平.结果 18例增殖期血管瘤mTOR、p70S6K-a的积分光密度分别为6336.47±1655.89,588.72±223.87;13例消退期血管瘤mTOR、p70S6K-a的积分光密度分别为846.22±297.09,3235.64±947.86;血管瘤增殖期p70S6K-a的积分光密度明显低于消退期,差异有统计学意义（P＜0.01）.血管瘤增殖期mTOR的积分光密度明显高于消退期,差异有统计学意义（P＜0.01）.结论 小儿血管瘤组织中有mTOR、p70S6K-α表达,mTOR/p70S6K信号通路可能在小儿血管瘤的病理演变中发挥作用.

Objective To investigate the role of mTOR/p70S6K signaling pathway in the development of hemangioma in children. Methods Hemangioma specimens from 31 patients without any other treatment except surgery were classified by Mulliken＇s classification. The expression of proliferating cell nuclear antigen （PCNA）, mammalian target of rapamycin （mTOR） and p70 ribosomal S6-kinase （p70S6K-α） were detected with immunohistochemistry. Results Eighteen cases of hemagioma specimens were classified as in proliferating phase, 13 as in involuting phase. For hemangiomas in proliferating phase, the IOD of mTOR and p70S6K-α were 6336. 47 ± 1655. 89 and 588. 72 ± 223. 87 respectively, while for hemagiomas in the involuting phase, the IOD of mTOR and p70S6K-α were 846. 22± 297. 09 and 3235. 64 ± 947. 86 respectively. IOD of p70S6K-α in hemangiomas in the involving phase was significantly higher than that in the proliferating phase （P＜0. 01 ）. IOD of mTOR in the hemagiomas in proliferating phase was significantly higher than that in the involuting phase （P＜0. 01 ）. Conclusions The mTOR/p70S6K signaling pathways may play an important role in growth and development of pediatric hemangioma.