新型钛合金2种不同表面处理对成骨细胞早期附着的影响

Adhesion of osteoblasts on the novel titanium alloy with bioactivity and wear coating

目的研究新型低弹性模量钛合金表面及其微弧氧化及耐磨处理后的表面形貌及粗糙度变化,以及不同表面处理的成骨细胞附着与2种涂层的表面形貌变化对细胞早期动力学行为影响特征。方法通过扫描电子显微镜(SEM)观察未处理组不同表面的形貌;采用SD新生鼠颅骨分离成骨细胞接种在材料表面并进行体外共同培养,MTT法检测30、60、120 min的不同表面的细胞早期附着量;细胞培养2 h后通过激光扫描共聚焦显微镜(CLSM)观察附着细胞的骨架蛋白结构。结果未处理组、耐磨与微弧氧化组材料表面的粗糙度值依次增加;细胞早期附着的吸光度A值随材料表面的粗糙度增加呈上升趋势,各组内3个时间点的细胞附着A值随时间延长而递增;2 h附着细胞骨架蛋白的量在微弧氧化、耐磨处理组表面表达均优于未处理组。结论 2种不同涂层处理后新合金表面的形貌及粗糙度发生改变,进而影响到细胞在其表面的早期附着的量及其形态、骨架蛋白的表达。

Objective The purpose of this study was to evaluate the rugosity with both of the bioactivity and the wear coating of a new type titanium implant material Ti-Zr-Sn-Mo-Nb（TZS）,and to investigate the adhesion of osteoblasts on different coating surface.Methods Both surface topography of bioactivity and wear coating were examined with scanning electron microscope（SEM）.The average roughness of two kinds coating were measured by portable roughometer.We examined the dynamic behavior of early adhesion of the osteoblasts,which were cultured on different coating surface of titanium alloy.As a marker of cell adhesions,cytoskeleton proteins were tagged by F-actin agent.Additionally,the number of adhesion cells was investigated by biological assay MTT.These results were compared with those founds in the control.Results Bioactivity-coating with pore structure and wear-coating with irregular pitting corrosion were observed.Roughometer examination revealed that the roughness of different disc was increased.The control bioactivity-coating surface was the most rough,followed by wear-coating and then the control.Bioactivity coating had best cell biocompatibility,in that the highest cell adhesion ratio was detected.It was very likely that bioactivity coating possessed of biomimetic character and roughness surface.Wear coating had similar biocompatible in comparison with bioactivity coating.The cytoskeleton protein of cell formed a fibrillar structure on bioactivity and wear coating.Cytoskeleton tensin was heterogeneously distributed on variety of surface depending on different surface topography.Conclusion The new titanium alloy shows rough surface after surface treatment,and then cell adhesion and morphology,cytoskeleton protein present wea on bioactivity-coating and wear-coating.